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AB321135

Alexa Fluor® 750 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

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Rabbit Recombinant Monoclonal VDAC1/Porin antibody - conjugated to Alexa Fluor® 750. Suitable for Target Binding Affinity, Antibody Labelling, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

VDAC, VDAC1, Non-selective voltage-gated ion channel VDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Non-selective voltage-gated ion channel VDAC2, VDAC-2, hVDAC2, Outer mitochondrial membrane protein porin 2, VDAC2

  • Carrier free

    Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - BSA and Azide free

  • Unconjugated

    Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

  • 660 APC

    APC Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control

  • HRP

    HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

  • 578 PE

    PE Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10852(B)

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

Target Binding Affinity, ICC/IF, Flow Cyt (Intra), Antibody Labelling, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Reagents, Controls & Accessories

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Primary Antibodies

AB8227

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

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Proteins & Peptides

AB132481

Recombinant Human VDAC1/Porin protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human VDAC1/Porin protein (GST tag N-Terminus) (AB132481)

  • 0
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Reagents, Controls & Accessories

AB110413

Total OXPHOS Rodent WB Antibody Cocktail

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

  • 5
  • 18
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VDAC1 also known as porin and VDAC2 are proteins found in the outer mitochondrial membrane. Both proteins facilitate the transfer of metabolites and ions across the mitochondrial membrane. VDAC1 has a molecular weight of approximately 31 kDa and VDAC2 is structurally similar. VDAC1 and VDAC2 are expressed in nearly all eukaryotic cells showing higher expression levels in tissues with high energy demands like the heart and brain. They serve as a pathway for communication between the mitochondria and the rest of the cell.
Biological function summary

These proteins play an important role in cell metabolism and energy homeostasis. VDAC1 and VDAC2 participate in the formation of a channel through the mitochondrial outer membrane linking the cytosol with the mitochondria. They are also part of larger complexes with proteins like hexokinase which binds to VDAC to regulate glycolytic energy metabolism. These interactions impact processes such as apoptosis and calcium ion regulation.

Pathways

These proteins are involved significantly in apoptosis and metabolic pathways. VDAC proteins interact with pro- and anti-apoptotic members of the Bcl-2 family like Bax and Bcl-xL influencing the release of cytochrome c during apoptosis. VDAC also plays a role in regulating metabolic pathways by interacting with proteins like creatine kinase which is important for high-energy phosphate metabolism. These interactions help maintain cellular homeostasis and respond to changing cellular energy needs.

VDAC1 and VDAC2 have significant roles. Alzheimer's disease and cancer show connections to altered function or expression of these proteins. In Alzheimer's VDAC1 interacts with amyloid-beta peptides affecting mitochondrial function and promoting neurodegeneration. In cancer alterations in VDAC1 expression can influence tumor metabolism and cell death resistance. Both diseases highlight interactions between VDAC proteins and other molecules that alter their function and contribute to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed : 10661876, PubMed : 11845315, PubMed : 18755977, PubMed : 30061676, PubMed : 8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed : 10661876, PubMed : 11845315, PubMed : 18755977, PubMed : 8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed : 10661876, PubMed : 18755977, PubMed : 8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed : 18755977, PubMed : 8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed : 18755977, PubMed : 31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed : 32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed : 15033708, PubMed : 25296756). May mediate ATP export from cells (PubMed : 30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed : 20230784).. Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.
See full target information VDAC1

Additional targets

Voltage-dependent anion-selective channel protein 2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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