JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB321161

Alexa Fluor® 750 Anti-WDR4 antibody [EPR11052]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal WDR4 antibody - conjugated to Alexa Fluor® 750.

View Alternative Names

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4, Protein Wuho homolog, WD repeat-containing protein 4, hWH, WDR4

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11052

Isotype

IgG

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The WDR4 protein also known as WD repeat domain 4 functions in the modification of ribonucleic acids. It specifically takes part in the methylation process of N(7)-methylguanine-tRNA (m7G46) where it serves as an important component of the tRNA methyltransferase complex. With an approximate mass of 51 kDa WDR4 shows expression in various tissues throughout the human body contributing to fundamental cellular processes.
Biological function summary

WDR4 plays a role in the maintenance of accurate protein synthesis. It contributes to the stabilization and correct folding of tRNA molecules which are essential for the translation of genetic information from mRNA to proteins. As part of the tRNA methyltransferase complex WDR4 interacts with the METTL1 enzyme highlighting its involvement in precise tRNA modification processes critical for cellular function and proliferation.

Pathways

The function of WDR4 integrates into the broader landscape of the RNA modification pathway. This pathway is tied to the regulation of gene expression and cellular homeostasis. WDR4's activity impacts the efficiency and fidelity of the translation pathway. In this context WDR4 works closely with METTL1 enhancing the stability and functional capacity of tRNA during protein synthesis which is pivotal for the correct transmission of genetic code within cells.

WDR4's malfunction or altered expression associates with several conditions including microcephalic primordial dwarfism where its disruption affects brain development and growth. The protein interacts with METTL1 implicated in the same disorder demonstrating the importance of their coordinated function. Additionally alterations in WDR4 expression might link to certain types of cancers where aberrant tRNA methylation affects tumor growth and progression indicating the potential for WDR4 as a therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed : 12403464, PubMed : 31031083, PubMed : 31031084, PubMed : 36599982, PubMed : 36599985, PubMed : 37369656). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding (PubMed : 36599982, PubMed : 36599985, PubMed : 37369656). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop (PubMed : 12403464, PubMed : 34352206, PubMed : 34352207, PubMed : 36599982, PubMed : 36599985, PubMed : 37369656). M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay (PubMed : 36599982, PubMed : 36599985). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (PubMed : 31031084, PubMed : 37379838). Also required for methylation of a specific subset of miRNAs, such as let-7 (PubMed : 31031083). Independently of METTL1, also plays a role in genome stability : localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed : 26751069).
See full target information WDR4
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB271063

Anti-METTL1 antibody [EPR24320-27]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL1 antibody [EPR24320-27] (AB271063)

  • 0
  • 0
Cell Lines & Lysates

AB258285

Human WDR4 knockout HeLa cell lysate

Western blot - Human WDR4 knockout HeLa cell lysate (AB258285)

  • 0
  • 0
Cell Lines & Lysates

AB265109

Human WDR4 knockout HeLa cell line

Advanced Validation

Western blot - Human WDR4 knockout HeLa cell line (AB265109)

  • 0
  • 0
Primary Antibodies

AB157300

PE/Cy5® Anti-CD3 antibody [UCHT1]

Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1] (AB157300)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com