Rabbit Polyclonal ALG1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALG1 aa 50-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ALG1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BT22
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
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Target data
Function
Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the first mannose residues to the dolichol-linked oligosaccharide chain, to produce Man1GlcNAc(2)-PP-dolichol core oligosaccharide. Man1GlcNAc(2)-PP-dolichol is a substrate for ALG2, the following enzyme in the biosynthetic pathway.
Alternative names
HMAT1, HMT1, PSEC0061, UNQ861/PRO1870, ALG1, Chitobiosyldiphosphodolichol beta-mannosyltransferase, Asparagine-linked glycosylation protein 1 homolog, GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-dolichol diphosphochitobiose mannosyltransferase, Mannosyltransferase-1, MT-1, hMat-1
Recommended products
Rabbit Polyclonal ALG1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALG1 aa 50-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ALG1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BT22
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ALG1 protein also known as chitobiosyldiphosphodolichol beta-mannosyltransferase plays an important role in the process of glycoprotein biosynthesis. It has a significant molecular mass of about 47 kDa. ALG1 is prominently expressed in the endoplasmic reticulum. Its function involves the catalysis of mannose transfer making it essential in the formation of glycan chains on proteins.
- Biological function summary
ALG1 participates in a larger enzymatic complex responsible for N-glycosylation. This process is important in modifying proteins for proper folding and stability. The protein integrates mannose into lipid-linked oligosaccharides which are then transferred to nascent proteins. ALG1 interacts closely with other enzymes in the glycosylation pathway ensuring the transfer of proper sugar moieties onto proteins.
- Pathways
ALG1 operates within the glycosylation and protein quality control pathways. The N-glycosylation pathway relies on ALG1 to add mannose units to dolichol-linked oligosaccharide precursors a necessary step for efficient protein folding. ALG1 links with proteins involved in the calnexin/calreticulin cycle which monitors and assists protein folding and assembly.
- Associated diseases and disorders
ALG1 mutations lead to Congenital Disorders of Glycosylation Type Ik (ALG1-CDG) which can cause severe developmental issues and multi-systemic problems. It interacts with proteins in the N-glycosylation process such as ALG2 and ALG5 which can also be implicated in glycosylation disorders. These disorders highlight the importance of ALG1 in maintaining proper cellular function and structure.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ALG1 antibody (ab154737)
7.5% SDS PAGE
All lanes: Western blot - Anti-ALG1 antibody (ab154737) at 1/500 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 53 kDa
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