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AB154737

Anti-ALG1 antibody

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(1 Publication)

Rabbit Polyclonal ALG1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALG1 aa 50-350.

View Alternative Names

HMAT1, HMT1, PSEC0061, UNQ861/PRO1870, ALG1, Chitobiosyldiphosphodolichol beta-mannosyltransferase, Asparagine-linked glycosylation protein 1 homolog, GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-dolichol diphosphochitobiose mannosyltransferase, Mannosyltransferase-1, MT-1, hMat-1

1 Images
Western blot - Anti-ALG1 antibody (AB154737)
  • WB

Unknown

Western blot - Anti-ALG1 antibody (AB154737)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ALG1 antibody (ab154737) at 1/500 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ALG1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q9BT22

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ALG1 protein also known as chitobiosyldiphosphodolichol beta-mannosyltransferase plays an important role in the process of glycoprotein biosynthesis. It has a significant molecular mass of about 47 kDa. ALG1 is prominently expressed in the endoplasmic reticulum. Its function involves the catalysis of mannose transfer making it essential in the formation of glycan chains on proteins.
Biological function summary

ALG1 participates in a larger enzymatic complex responsible for N-glycosylation. This process is important in modifying proteins for proper folding and stability. The protein integrates mannose into lipid-linked oligosaccharides which are then transferred to nascent proteins. ALG1 interacts closely with other enzymes in the glycosylation pathway ensuring the transfer of proper sugar moieties onto proteins.

Pathways

ALG1 operates within the glycosylation and protein quality control pathways. The N-glycosylation pathway relies on ALG1 to add mannose units to dolichol-linked oligosaccharide precursors a necessary step for efficient protein folding. ALG1 links with proteins involved in the calnexin/calreticulin cycle which monitors and assists protein folding and assembly.

ALG1 mutations lead to Congenital Disorders of Glycosylation Type Ik (ALG1-CDG) which can cause severe developmental issues and multi-systemic problems. It interacts with proteins in the N-glycosylation process such as ALG2 and ALG5 which can also be implicated in glycosylation disorders. These disorders highlight the importance of ALG1 in maintaining proper cellular function and structure.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the first mannose residues to the dolichol-linked oligosaccharide chain, to produce Man1GlcNAc(2)-PP-dolichol core oligosaccharide. Man1GlcNAc(2)-PP-dolichol is a substrate for ALG2, the following enzyme in the biosynthetic pathway.
See full target information ALG1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 10:579 PubMed35722357

2022

Long non-coding RNA (FALEC) promotes malignant behaviors of gastric cancer cells by regulating miR-203b/PIM3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Dong,Mancheng Gong,Jianjun Xiao,Huifen Li,Muyou Tian,Senming Wang
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Product promise

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