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AB155935

Anti-ALG1 antibody

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Rabbit Polyclonal ALG1 antibody. Suitable for WB and reacts with Caenorhabditis elegans samples.

View Alternative Names

4-mannosyltransferase, ALG1_HUMAN, Asparagine-linked glycosylation protein 1 homolog, Beta 1 4 mannosyltransferase, Beta-1, CDG1K, Chitobiosyldiphosphodolichol beta-mannosyltransferase, GDP Man GlcNAc2 PP dolichol mannosyltransferase, GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase, GDP-mannose-dolichol diphosphochitobiose mannosyltransferase, HMT 1, Hmat-1, MT-1, Mannosyltransferase-1, asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase), asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)

1 Images
Western blot - Anti-ALG1 antibody (AB155935)
  • WB

Unknown

Western blot - Anti-ALG1 antibody (AB155935)

4-20% Tris-HCl polyacrylamide gel.

All lanes:

Western blot - Anti-ALG1 antibody (ab155935) at 1/5000 dilution

Lane 1:

Wild type Caenorhabditis elegans extract at 15 µg

Lane 2:

ALG1 negative (-) Caenorhabditis elegans extract at 15 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/15000 dilution

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Caenorhabditis elegans

Applications

WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Caenorhabditis elegans": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ALG1 protein also known as chitobiosyldiphosphodolichol beta-mannosyltransferase plays an important role in the process of glycoprotein biosynthesis. It has a significant molecular mass of about 47 kDa. ALG1 is prominently expressed in the endoplasmic reticulum. Its function involves the catalysis of mannose transfer making it essential in the formation of glycan chains on proteins.
Biological function summary

ALG1 participates in a larger enzymatic complex responsible for N-glycosylation. This process is important in modifying proteins for proper folding and stability. The protein integrates mannose into lipid-linked oligosaccharides which are then transferred to nascent proteins. ALG1 interacts closely with other enzymes in the glycosylation pathway ensuring the transfer of proper sugar moieties onto proteins.

Pathways

ALG1 operates within the glycosylation and protein quality control pathways. The N-glycosylation pathway relies on ALG1 to add mannose units to dolichol-linked oligosaccharide precursors a necessary step for efficient protein folding. ALG1 links with proteins involved in the calnexin/calreticulin cycle which monitors and assists protein folding and assembly.

ALG1 mutations lead to Congenital Disorders of Glycosylation Type Ik (ALG1-CDG) which can cause severe developmental issues and multi-systemic problems. It interacts with proteins in the N-glycosylation process such as ALG2 and ALG5 which can also be implicated in glycosylation disorders. These disorders highlight the importance of ALG1 in maintaining proper cellular function and structure.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ALG1
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Product promise

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