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AB250071

Anti-ALG2 antibody [EPR12006(2)(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal ALG2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

UNQ666/PRO1298, ALG2, Asparagine-linked glycosylation protein 2 homolog, GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase

1 Images
Western blot - Anti-ALG2 antibody [EPR12006(2)(B)] - BSA and Azide free (AB250071)
  • WB

Supplier Data

Western blot - Anti-ALG2 antibody [EPR12006(2)(B)] - BSA and Azide free (AB250071)

This data was developed using ab178697, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ALG2 antibody [EPR12006(2)(B)] (<a href='/en-us/products/primary-antibodies/alg2-antibody-epr120062b-ab178697'>ab178697</a>) at 1/10000 dilution

Lane 1:

HepG2 lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

A431 lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12006(2)(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250071 is the carrier-free version of ab178697.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ALG2 protein also known as asparagine-linked glycosylation 2 plays a role in the early steps of N-glycan precursor biosynthesis. ALG2 has a molecular mass of approximately 51 kDa and shows expression in multiple tissues including the liver and brain. The protein acts mechanically to facilitate the addition of the first mannose residue to the lipid-linked oligosaccharide (LLO) precursor in the endoplasmic reticulum a fundamental step in protein glycosylation processes.
Biological function summary

Certain processes depend on the actions of ALG2. This protein becomes part of the larger oligosaccharyltransferase complex where it ensures proper glycosylation of nascent proteins—a modification important for protein folding and stability. ALG2 modulates the efficiency of protein maturation by its role in glycan formation affecting how proteins interact within cellular processes. Defects in ALG2 lead to improper protein folding and cellular distribution.

Pathways

ALG2 functions as an important component in the protein glycosylation pathway and more specifically in the N-glycan assembly pathway. This protein interacts closely with other glycosylation enzymes like ALG12 which further add branches to the glycans once ALG2 completes its function. The interactions within this pathway ensure proper biological functionalization of glycoproteins facilitating diverse roles from cell signaling to immune recognition.

ALG2 mutations have known associations with congenital disorders of glycosylation (CDG) a group of diseases caused by defects in glycosylation. Such mutations can cause a spectrum of developmental and metabolic issues. ALG2 dysfunction linked to proteins like ALG9 in co-regulatory roles reveals the impact of glycosylation on cellular homeostasis. These insights into ALG2 give promise for potential therapeutic targets in managing CDG symptoms and advancing understanding of glycosylation-related pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the second and third mannose residues to the dolichol-linked oligosaccharide chain, to produce Man3GlcNAc(2)-PP-dolichol core oligosaccharide. Man3GlcNAc(2)-PP-dolichol is a substrate for ALG11, the following enzyme in the biosynthetic pathway (PubMed : 12684507, PubMed : 35136180). While both alpha 1,3 and alpha 1,6 linkages are possible, the sequential addition of alpha 1,3 followed by alpha 1,6 is probably the preferred route (PubMed : 35136180).
See full target information ALG2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com