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AB151211

Anti-ALG3 antibody

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(3 Publications)

Rabbit Polyclonal ALG3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALG3 aa 50-150.

View Alternative Names

NOT, NOT56L, ALG3, Asparagine-linked glycosylation protein 3 homolog, Dol-P-Man-dependent alpha(1-3)-mannosyltransferase, Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase, Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase, Not56-like protein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALG3 antibody (AB151211)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALG3 antibody (AB151211)

Paraffin embedded human testis tissue stained for ALG3 using ab151211 at 1/200 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALG3 antibody (AB151211)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALG3 antibody (AB151211)

Paraffin embedded human gastrointestinal tissue stained for ALG3 using ab151211 at 1/200 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALG3 antibody (AB151211)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALG3 antibody (AB151211)

Paraffin embedded human kidney tissue stained for ALG3 using ab151211 at 1/200 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALG3 antibody (AB151211)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALG3 antibody (AB151211)

Paraffin embedded human placenta tissue stained for ALG3 using ab151211 at 1/200 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ALG3 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q92685

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALG3 also known as alpha-13-mannosyltransferase is an enzyme with a role in the glycosylation process. It is encoded by the ALG3 gene and has a molecular mass of approximately 48 kDa. ALG3 is mainly expressed in the endoplasmic reticulum where it performs its functions in protein modification. This enzyme catalyzes the addition of mannose to lipid-linked oligosaccharide precursors an important step in N-glycan biosynthesis. Its activity and expression are important for the proper folding and quality control of glycoproteins.
Biological function summary

ALG3 functions as an important player in the N-glycosylation pathway. It acts as part of an essential enzymatic complex that modifies the lipid-linked oligosaccharide. This modification is necessary for protein maturation and stability as glycosylation is a post-translational modification influencing protein folding and stability. Dysfunction in this process can result in incorrect protein folding leading to cellular stress or dysfunction.

Pathways

ALG3 integrates into the N-glycan biosynthesis pathway a vital cellular process. This pathway involves a series of enzymatic steps important for generating glycoproteins. ALG3 interacts directly with other glycosyltransferases such as ALG1 and ALG2 as part of this biosynthetic pathway. Cooperation among these enzymes ensures the correct assembly and elongation of glycan structures which are important for a variety of cellular functions.

ALG3 is associated with Congenital Disorders of Glycosylation (CDG). These are genetic disorders that result from mutations in genes involved in the glycosylation process leading to a wide range of symptoms and impairment. Specific malfunction of ALG3 can disrupt normal glycan structure and protein glycosylation causing severe developmental and physiological issues. This disorder highlights the significance of ALG3 in maintaining proper cellular and systemic function through its interaction with other proteins in glycosylation pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dol-P-Man : Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol (PubMed : 10581255). Man(6)GlcNAc(2)-PP-dolichol is a substrate for ALG9, the following enzyme in the biosynthetic pathway (PubMed : 10581255).
See full target information ALG3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 40:149 PubMed33931075

2021

ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Sun,Zhenyu He,Ling Guo,Caiqin Wang,Chuyong Lin,Liping Ye,Xiaoqing Wang,Yue Li,Meisongzhu Yang,Sailan Liu,Xin Hua,Wen Wen,Chao Lin,Zhiqing Long,Wenwen Zhang,Han Li,Yunting Jian,Ziyuan Zhu,Xianqiu Wu,Huanxin Lin

Cell death & disease 9:688 PubMed29880818

2018

Aberrant mannosylation profile and FTX/miR-342/ALG3-axis contribute to development of drug resistance in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Xiaolu Ma,Qianqian Liu,Yang Xiao,Shimeng Pan,Li Jia

Medical science monitor : international medical journal of experimental and clinical research 24:3479-3487 PubMed29799832

2018

ALG3 Is Activated by Heat Shock Factor 2 and Promotes Breast Cancer Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yongde Yang,Yanlin Zhou,Xin Xiong,Man Huang,Xueyan Ying,Mengyuan Wang
View all publications
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