Anti-ALIX antibody [3A9] (ab117600) is a mouse monoclonal antibody that is used to detect ALIX in Western Blot. Suitable for Human samples.
- Over 100 publications
Images
![Western blot - Anti-ALIX antibody [3A9] (AB117600), expandable thumbnail](https://content.abcam.com/products/images/alix-antibody-3a9-ab117600--western-blot-img154010.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALIX antibody [3A9] (AB117600)")
Publications
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- Experimental & molecular medicine 55:1399-14122023Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBasalova Nataliya,Arbatskiy Mikhail,Popov Vladimir,Grigorieva Olga,Vigovskiy Maksim,Zaytsev Ivan,Novoseletskaya Ekaterina,Sagaradze Georgy,Danilova Natalia,Malkov Pavel,Cherniaev Andrey,Samsonova Maria,Karagyaur Maxim,Tolstoluzhinskaya Anastasiya,Dyachkova Uliana,Akopyan Zhanna,Tkachuk Vsevolod,Kalinina Natalia,Efimenko AnastasiyaPubMed 37394579
- Cellular and molecular life sciences : CMLS 80:1772023siRNA screening reveals that SNAP29 contributes to exosome release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNina Pettersen Hessvik,Krizia Sagini,Silvana Romero,Manuel Ramirez-Garrastacho,Marta Rodriguez,Astrid Elisabeth V Tutturen,Audun Kvalvaag,Espen Stang,Andreas Brech,Kirsten Sandvig,Alicia LlorentePubMed 37285022
- Scientific reports 13:77662023Ceramide present in cholangiocarcinoma-derived extracellular vesicle induces a pro-inflammatory state in monocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Oliviero,Michele Dei Cas,Aida Zulueta,Roberta Maiello,Alessandro Villa,Carla Martinelli,Elena Del Favero,Monica Falleni,Linda Montavoci,Stefania Varchetta,Dalila Mele,Matteo Donadon,Cristiana Soldani,Barbara Franceschini,Marcello Maestri,Gaetano Piccolo,Matteo Barabino,Paolo Pietro Bianchi,Jesus M Banales,Stefania Mantovani,Mario U Mondelli,Anna CarettiPubMed 37173330
- Frontiers in nutrition 10:11626792023The RNA cargo in small extracellular vesicles from chicken eggs is bioactive in C57BL/6 J mice and human peripheral blood mononuclear cells .Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeborah Fratantonio,Javaria Munir,Jiang Shu,Katherine Howard,Scott R Baier,Juan Cui,Janos ZempleniPubMed 37305095
- Cellular oncology (Dordrecht) 46:909-9312023Small extracellular vesicles promote invadopodia activity in glioblastoma cells in a therapy-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClarissa A Whitehead,Haoyun Fang,Huaqi Su,Andrew P Morokoff,Andrew H Kaye,Eric Hanssen,Cameron J Nowell,Katharine J Drummond,David W Greening,Laura J Vella,Theo Mantamadiotis,Stanley S StylliPubMed 37014551
- Materials today. Bio 16:1004252022A tannic acid doped hydrogel with small extracellular vesicles derived from mesenchymal stem cells promotes spinal cord repair by regulating reactive oxygen species microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhong Liu,Song Guo,Lanlan Dong,Peipei Wu,Kewei Li,Xinhua Li,Xiang Li,Hui Qian,Qiang FuPubMed 36186847
- Frontiers in cellular and infection microbiology 12:8507442022Anti-Viral Activities of Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Against Human Respiratory Viruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoo-Jin Oh,Eun-Na Lee,Joo-Hoo Park,Jae Kyung Lee,Geum Joon Cho,Il-Ho Park,Ok Sarah ShinPubMed 35558099
- PloS one 17:e02671642022Extracellular vesicles enriched with amylin receptor are cytoprotective against the Aß toxicity in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRania Soudy,Ryoichi Kimura,Wen Fu,Aarti Patel,Jack JhamandasPubMed 35421203
- Experimental and therapeutic medicine 23:3822022Altered small non-coding RNA expression profiles of extracellular vesicles in the prostatic fluid of patients with chronic pelvic pain syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Ouyang,Dayu Han,Zexin Guo,Junhong Deng,Weilong Li,Liangliang Huang,Jianming Liu,Zhouda Cai,Jun Bian,Shaoming HuangPubMed 35495611
- Journal of neuroinflammation 19:802022Extracellular vesicles derived from mesenchymal stem cells alleviate neuroinflammation and mechanical allodynia in interstitial cystitis rats by inhibiting NLRP3 inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi Zhang,Yong Huang,Fubing Ouyang,Minzhi Su,Wenbiao Li,Jialiang Chen,Hengjun Xiao,Xiangfu Zhou,Bolong LiuPubMed 35387668
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Protein within Human PDCD6IP aa 600-750. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WUM4
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
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Target data
Function
Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed:14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17556548, PubMed:17853893). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17556548, PubMed:17853893, PubMed:18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity). (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).
Alternative names
AIP1, ALIX, KIAA1375, PDCD6IP, Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95
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Anti-ALIX antibody [3A9] (ab117600) is a mouse monoclonal antibody that is used to detect ALIX in Western Blot. Suitable for Human samples.
- Over 100 publications
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Protein within Human PDCD6IP aa 600-750. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WUM4
- Clone number
- 3A9
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ALIX antibody [3A9] (ab117600) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.
What is the molecular weight of ALIX?
Anti-ALIX [3A9] (ab117600) specifically detects a band for ALIX (UniProt: Q8WUM4) at a molecular weight of 96kDa.
Trusted by the scientific community
Anti-ALIX [3A9] (ab117600) was first used in a scientific publication in 2011 and has been cited over 100 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALIX also known as PDCD6IP or ALIX G is an adapter protein involved in various cellular processes. It holds a molecular weight of approximately 95-100 kDa and is widely expressed in different tissue types including HEK 293 cells. ALIX plays a significant role as a component of the endosomal sorting complexes required for transport (ESCRT) machinery contributing to membrane dynamics and intracellular trafficking.
- Biological function summary
ALIX acts as an important mediator in the regulation of cellular membrane remodeling. It associates with several protein complexes influencing the budding and fission of vesicles and endosomes. The protein also interacts with ubiquitin and other factors guiding protein sorting and maintaining cellular homeostasis. This adapter characteristic makes ALIX essential for recycling receptors and maintaining cell surface receptor expression levels.
- Pathways
ALIX is involved in the ESCRT pathway and the apoptotic pathways. It works closely with proteins like TSG101 and CHMP4 to facilitate vesicle formation and dispatch impacting cellular processes like signaling and waste disposal. Furthermore ALIX regulates apoptosis by interacting with proteins such as ALG-2 and to some extent with apoptosis-related membranes to mediate programmed cell death.
- Associated diseases and disorders
ALIX has established links to cancer progression and neurodegenerative disorders. Its participation in membrane remodeling and protein sorting correlates with tumor development with evidence showing that altered expression affects oncogenic pathways. Additionally dysregulation of ALIX-associated pathways has implications in neurodegenerative diseases where ALIX and proteins such as those in the ESCRT complex could play a role in the degradation of misfolded proteins and endocytic dysfunctions contributing to disease pathology.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-ALIX antibody [3A9] (ab117600), expandable thumbnail](https://content.abcam.com/products/images/alix-antibody-3a9-ab117600--western-blot-img154010.jpg "Western blot - Anti-ALIX antibody [3A9] (ab117600)")
Western blot - Anti-ALIX antibody [3A9] (ab117600)
All lanes: Western blot - Anti-ALIX antibody [3A9] (ab117600)
All lanes: HeLa whole cell lysate
SecondaryAll lanes: Rabbit Anti Mouse IgG
Predicted band size: 96 kDa
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