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Rabbit Recombinant Monoclonal ALIX antibody. Carrier free. Suitable for WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed:14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17853893, PubMed:17556548). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17853893, PubMed:17556548, PubMed:18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).(Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).
Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95, ALIX, KIAA1375, AIP1, PDCD6IP
Rabbit Recombinant Monoclonal ALIX antibody. Carrier free. Suitable for WB and reacts with Human samples.
Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95, ALIX, KIAA1375, AIP1, PDCD6IP
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR15314-33
Affinity purification Protein A
Blue Ice
+4°C
Do Not Freeze
ab226768 is the carrier-free version of ab186728.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
ALIX acts as an important mediator in the regulation of cellular membrane remodeling. It associates with several protein complexes influencing the budding and fission of vesicles and endosomes. The protein also interacts with ubiquitin and other factors guiding protein sorting and maintaining cellular homeostasis. This adapter characteristic makes ALIX essential for recycling receptors and maintaining cell surface receptor expression levels.
ALIX also known as PDCD6IP or ALIX G is an adapter protein involved in various cellular processes. It holds a molecular weight of approximately 95-100 kDa and is widely expressed in different tissue types including HEK 293 cells. ALIX plays a significant role as a component of the endosomal sorting complexes required for transport (ESCRT) machinery contributing to membrane dynamics and intracellular trafficking.
ALIX is involved in the ESCRT pathway and the apoptotic pathways. It works closely with proteins like TSG101 and CHMP4 to facilitate vesicle formation and dispatch impacting cellular processes like signaling and waste disposal. Furthermore ALIX regulates apoptosis by interacting with proteins such as ALG-2 and to some extent with apoptosis-related membranes to mediate programmed cell death.
ALIX has established links to cancer progression and neurodegenerative disorders. Its participation in membrane remodeling and protein sorting correlates with tumor development with evidence showing that altered expression affects oncogenic pathways. Additionally dysregulation of ALIX-associated pathways has implications in neurodegenerative diseases where ALIX and proteins such as those in the ESCRT complex could play a role in the degradation of misfolded proteins and endocytic dysfunctions contributing to disease pathology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186728).
All lanes: Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (AB186728) at 1/10000 dilution
Lane 1: HEK-293 whole cell lysate at 20 µg
Lane 2: K562 whole cell lysate at 20 µg
Lane 3: Jurkat whole cell lysate at 20 µg
Lane 4: HeLa whole cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 96 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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