JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB186728

Anti-ALIX antibody [EPR15314-33] - N-terminal

4

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal ALIX antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

AIP1, ALIX, KIAA1375, PDCD6IP, Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95

2 Images
Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (AB186728)
  • WB

Lab

Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (AB186728)

Lanes 1 - 3 : Merged signal (red and green). Green - ab186728 observed at 96 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab186728 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in ALIX knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ALIX knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab186728 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728) at 1/5000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

ALIX knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PDCD6IP knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-pdcd6ip-knockout-hek-293-cell-line-ab260864'>ab260864</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 96 kDa

Observed band size: 96 kDa

false

Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (AB186728)
  • WB

Supplier Data

Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (AB186728)

All lanes:

Western blot - Anti-ALIX antibody [EPR15314-33] - N-terminal (ab186728) at 1/10000 dilution

Lane 1:

HEK-293 whole cell lysate at 20 µg

Lane 2:

K562 whole cell lysate at 20 µg

Lane 3:

Jurkat whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 96 kDa

false

  • Carrier free

    Anti-ALIX antibody [EPR15314-33] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15314-33

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB289653

Human ALIX ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALIX also known as PDCD6IP or ALIX G is an adapter protein involved in various cellular processes. It holds a molecular weight of approximately 95-100 kDa and is widely expressed in different tissue types including HEK 293 cells. ALIX plays a significant role as a component of the endosomal sorting complexes required for transport (ESCRT) machinery contributing to membrane dynamics and intracellular trafficking.
Biological function summary

ALIX acts as an important mediator in the regulation of cellular membrane remodeling. It associates with several protein complexes influencing the budding and fission of vesicles and endosomes. The protein also interacts with ubiquitin and other factors guiding protein sorting and maintaining cellular homeostasis. This adapter characteristic makes ALIX essential for recycling receptors and maintaining cell surface receptor expression levels.

Pathways

ALIX is involved in the ESCRT pathway and the apoptotic pathways. It works closely with proteins like TSG101 and CHMP4 to facilitate vesicle formation and dispatch impacting cellular processes like signaling and waste disposal. Furthermore ALIX regulates apoptosis by interacting with proteins such as ALG-2 and to some extent with apoptosis-related membranes to mediate programmed cell death.

ALIX has established links to cancer progression and neurodegenerative disorders. Its participation in membrane remodeling and protein sorting correlates with tumor development with evidence showing that altered expression affects oncogenic pathways. Additionally dysregulation of ALIX-associated pathways has implications in neurodegenerative diseases where ALIX and proteins such as those in the ESCRT complex could play a role in the degradation of misfolded proteins and endocytic dysfunctions contributing to disease pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed : 14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed : 17556548, PubMed : 17853893). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed : 17556548, PubMed : 17853893, PubMed : 18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed : 22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).. (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).
See full target information PDCD6IP
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23152 PubMed37603538

2023

Exosomal circBBS2 inhibits ferroptosis by targeting miR-494 to activate SLC7A11 signaling in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Hong,Tingting Zhao,Wei He,Jian Xia,Qing Huang,Jie Yang,Wenping Gu,Changqing Chen,Ning Zhang,Yunhai Liu,Jie Feng

Scientific reports 12:10851 PubMed35761023

2022

A comparative analysis of extracellular vesicles (EVs) from human and feline plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Jane Howard,Kieran Wynne,Evelin Moldenhauer,Paul Clarke,Ciaran Maguire,Stephanie Bollard,Xiaofei Yin,Lorraine Brennan,Louise Mooney,Stephen Fitzsimons,Melinda Halasz,Ester Rani Aluri,Dermot F Brougham,Walter Kolch,Róisín M Dwyer,Shirley Potter,Pamela Kelly,Amanda McCann

Cell death & disease 11:1008 PubMed33230102

2020

Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-κB/MMP-2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shoujun Bai,Xiaoyan Xiong,Bo Tang,Tingting Ji,Xiaoying Li,Xiaolei Qu,Weiliang Li

Cancers 12: PubMed31877768

2019

miR-10a-5p and miR-29b-3p as Extracellular Vesicle-Associated Prostate Cancer Detection Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Stefan Worst,Christopher Previti,Katja Nitschke,Nicolle Diessl,Julia Christina Gross,Lena Hoffmann,Lisa Frey,Vanessa Thomas,Christoph Kahlert,Karen Bieback,Adriana Torres Crigna,Fabia Fricke,Stefan Porubsky,Niklas Westhoff,Jost von Hardenberg,Philipp Nuhn,Philipp Erben,Maurice Stephan Michel,Michael Boutros

Journal of extracellular vesicles 8:1670935 PubMed31632620

2019

Transcriptomic profiling of cell-free and vesicular microRNAs from matched arterial and venous sera.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Hermann,Dominik Buschmann,Benedikt Kirchner,Melanie Borrmann,Florian Brandes,Stefan Kotschote,Michael Bonin,Anja Lindemann,Marlene Reithmair,Gustav Schelling,Michael W Pfaffl

Biomolecular detection and quantification 17:100089 PubMed31194192

2019

Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Mussack,Georg Wittmann,Michael W Pfaffl
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB117600

Anti-ALIX antibody [3A9]

primary-antibodies

alix-antibody-3a9-ab117600

4

(3 reviews)

Alternative Product
Primary Antibodies

AB88743

Anti-ALIX antibody

primary-antibodies

alix-antibody-ab88743

4

(2 reviews)

Alternative Product
Primary Antibodies

AB225555

Anti-ALIX antibody

primary-antibodies

alix-antibody-ab225555

0

(0 reviews)

Alternative Product
Primary Antibodies

AB88388

Anti-ALIX antibody

primary-antibodies

alix-antibody-ab88388

5

(2 reviews)

Alternative Product
Primary Antibodies

AB76608

Anti-ALIX antibody

primary-antibodies

alix-antibody-ab76608

0

(0 reviews)

Alternative Product
Primary Antibodies

AB186429

Anti-ALIX antibody [EPR15314] - N-terminal

primary-antibodies

alix-antibody-epr15314-n-terminal-ab186429

4

(3 reviews)

Alternative Product
Primary Antibodies

AB232611

Anti-ALIX antibody [EPR15314] - BSA and Azide free

primary-antibodies

alix-antibody-epr15314-bsa-and-azide-free-ab232611

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com