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AB186429

Anti-ALIX antibody [EPR15314] - N-terminal

4

(3 Reviews)

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(145 Publications)

Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) is a rabbit monoclonal antibody detecting ALIX in Western Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 80 publications

View Alternative Names

AIP1, ALIX, KIAA1375, PDCD6IP, Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95

2 Images
Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (AB186429)
  • WB

Supplier Data

Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (AB186429)

All lanes:

Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 96 kDa

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Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (AB186429)
  • WB

Supplier Data

Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (AB186429)

All lanes:

Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 96 kDa

false

  • Carrier free

    Anti-ALIX antibody [EPR15314] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15314

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/240", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/240", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/240", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of ALIX?
Anti-ALIX [EPR15314] - N-terminal (ab186429) specifically detects a band for ALIX (UniProt: Q8WUM4) at a molecular weight of 97kDa.

Trusted by the scientific community
Anti-ALIX [EPR15314] - N-terminal (ab186429) was first used in a scientific publication in 2014 and has been cited over 80 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR15314] also available for your convenience: ab186429, Carrier free - ab232611

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALIX also known as PDCD6IP or ALIX G is an adapter protein involved in various cellular processes. It holds a molecular weight of approximately 95-100 kDa and is widely expressed in different tissue types including HEK 293 cells. ALIX plays a significant role as a component of the endosomal sorting complexes required for transport (ESCRT) machinery contributing to membrane dynamics and intracellular trafficking.
Biological function summary

ALIX acts as an important mediator in the regulation of cellular membrane remodeling. It associates with several protein complexes influencing the budding and fission of vesicles and endosomes. The protein also interacts with ubiquitin and other factors guiding protein sorting and maintaining cellular homeostasis. This adapter characteristic makes ALIX essential for recycling receptors and maintaining cell surface receptor expression levels.

Pathways

ALIX is involved in the ESCRT pathway and the apoptotic pathways. It works closely with proteins like TSG101 and CHMP4 to facilitate vesicle formation and dispatch impacting cellular processes like signaling and waste disposal. Furthermore ALIX regulates apoptosis by interacting with proteins such as ALG-2 and to some extent with apoptosis-related membranes to mediate programmed cell death.

ALIX has established links to cancer progression and neurodegenerative disorders. Its participation in membrane remodeling and protein sorting correlates with tumor development with evidence showing that altered expression affects oncogenic pathways. Additionally dysregulation of ALIX-associated pathways has implications in neurodegenerative diseases where ALIX and proteins such as those in the ESCRT complex could play a role in the degradation of misfolded proteins and endocytic dysfunctions contributing to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed : 14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed : 17556548, PubMed : 17853893). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed : 17556548, PubMed : 17853893, PubMed : 18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed : 22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity).. (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses).
See full target information PDCD6IP
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Publications (145)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 47: PubMed41020812

2025

TGF-β-Enriched Exosomes from Acute Myeloid Leukemia Activate Smad2/3-MMP2 and ERK1/2 Signaling to Promote Leukemic Cell Proliferation, Migration, and Immune Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Jia

Biological procedures online 27:33 PubMed40836218

2025

Refining Flow Cytometry-based Sorting of Plasma-derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Reverberi,Maria Chiara Ciferri,Nicole Rosenwasser,Alessandro Catino,Giuseppina Poppa,Ilaria Giusti,Vincenza Dolo,Rodolfo Quarto,Sara Santamaria,Monica Colombo,Simona Coco,Roberta Tasso

Redox biology 86:103826 PubMed40825268

2025

Macrophages and macrophage extracellular vesicles confer cancer ferroptosis resistance via PRDX6-mediated mitophagy inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Naisheng Zheng,Fuli Li,Qing Huang,Xian Huang,Tomasz Maj

Journal of cellular and molecular medicine 29:e70725 PubMed40770945

2025

Deciphering the Proteomic Landscape of Circulating Extracellular Vesicles in Human Abdominal Aortic Aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyang Yu,Ge Zhang,Shaotong Pei,Yifei Zhang,Peiyu Yuan,Renying Miao,Kaisaierjiang Kadier,Pengpeng Zhang,Tianshu Gu,Ruhao Wu,Haonan Zhang,Shiqian Zhang,Bo Yang,Han Wu,Yudi Xu,Ke Hu,Qingfei Xu,Yaxin Chen,Jinliang Wang,Zongao Cai,Junnan Tang,Teng Li,Yan Song

Oncology letters 30:444 PubMed40740986

2025

Proteomics analysis reveals elevated RAB21 in serum-derived extracellular vesicles from patients with follicular thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kyojiro Kawakami,Naoki Edo,Koji Morita,Toshio Ishikawa,Hiroyuki Onose,Tatsuya Fukumori,Hiroki Tsumoto,Keitaro Umezawa,Masafumi Ito,Yuri Miura

Frontiers in molecular neuroscience 18:1588007 PubMed40547480

2025

Non-invasive biomarkers for brain aging: the role of autophagy-related microRNAs in plasma exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Cheng,Shuyi Yu,Zhikang Cui,Hang Chen,Jing Fan,Qian Yu,Yan Jin,Yunshan Wang,Ming Li,Zhiming Lu

Journal of nanobiotechnology 23:439 PubMed40514650

2025

Nanoscale engineered exosomes for dual delivery of Sirtuin3 and insulin to ignite mitochondrial recovery in myocardial ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Yang,Xinyi Yun,Weihan Zheng,Huihui Zhang,Zi Yan,Youyu Chen,Wanting Xue,Siqi Mi,Ziyue Li,Hanxiao Sun,Guozhi Xiao,Zhenning Dai,Shiyu Li,Wenhua Huang

Stem cell research & therapy 16:282 PubMed40462173

2025

Exosomal miR-202-5p derived from iPSC-MSCs protects against myocardial infarction through inhibition of cardiomyocyte pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Chen,Xiaoting Liang,Qian Han,Haiwei He,Xinran Huang,Ying Shen,Jie Qiu,Fang Lin,Cong Mai,Ziqi Li,Kexin Ma,Bei Hu,Xin Li,Yuelin Zhang

Journal of extracellular biology 4:e70054 PubMed40453705

2025

Scalable, High-Throughput Isolation of Extracellular Vesicles Using Electrokinetic-Assisted Mesh Filtration: ExoFilter.

Applications

Unspecified application

Species

Unspecified reactive species

KangMin Lee,Minju Bae,YongWoo Kim,SoYoung Jeon,Sujin Kang,Wonjong Rhee,Sehyun Shin

Bioengineering & translational medicine 10:e10743 PubMed40385541

2025

Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Emily H Powsner,Stephanie M Kronstadt,Kristin Nikolov,Amaya Aranda,Steven M Jay
View all publications
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