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AB263863

Anti-ALK-1 antibody [EPR22985-125]

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Rabbit Recombinant Monoclonal ALK-1 antibody. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

Acvrlk1, Alk-1, Acvrl1, Activin receptor type-1-like, Activin receptor-like kinase 1, Serine/threonine-protein kinase receptor R3, TGF-B superfamily receptor type I, ALK-1, SKR3, TSR-I

1 Images
Flow Cytometry - Anti-ALK-1 antibody [EPR22985-125] (AB263863)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ALK-1 antibody [EPR22985-125] (AB263863)

Flow cytometric analysis of MEF (Mouse embryonic fibroblast (immortalized)) cells (Left) / bEnd.3 (mouse brain endothelioma) cells (Right) cells labelling ALK-1 with ab263863 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

Negative control : MEF (PMID : 11927558).

Gated on viable cells.

  • Carrier free

    Anti-ALK-1 antibody [EPR22985-125] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22985-125

Isotype

IgG1

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALK-1 also known as ACVRL1 or ALK1 protein is a receptor serine/threonine kinase with a molecular mass of approximately 55 kDa. It plays an important role in the TGF-beta superfamily signaling particularly in angiogenesis and blood vessel development. Expressed mainly in endothelial cells ALK-1 mediates cellular responses essential for maintaining vascular stability. The careful balance of its signaling is necessary indicating its importance in physiological conditions.
Biological function summary

The function of ALK-1 extends to the formation and maintenance of vascular endothelium as part of the larger receptor complex. It interacts with ligands such as BMP9 and BMP10 which regulate endothelial cell proliferation and differentiation. ALK-1's role as part of this receptor complex highlights its involvement in controlling the fine balance between angiogenic and anti-angiogenic processes. Its biological activity is vital for healthy vascular development and homeostasis.

Pathways

ALK-1 activation is a part of the BMP signaling pathway a branch of the larger TGF-beta pathway. This pathway is significant in various cellular processes including growth and differentiation. ALK-1 interacts with other signalling mediators like SMAD1/5/8 proteins which translocate to the nucleus to affect gene expression. Connecting with these pathways ALK-1 aligns closely with ACVR2A and ENG (Endoglin) which further highlight its role in vascular biology.

ALK-1 mutations or dysregulation leads to conditions such as hereditary hemorrhagic telangiectasia (HHT) and pulmonary arterial hypertension (PAH). These conditions showcase the important role ALK-1 plays in vascular integrity and signaling. ALK-1 is connected to disorders through interactions with proteins such as ENG further highlighting its involvement in vascular-related pathologies. Maintaining precise ALK-1 function is pivotal to preventing these disorders indicating its potential as a therapeutic target or biomarker.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
See full target information Acvrl1
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Alternative Products

Primary Antibodies

AB51870

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Primary Antibodies

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Primary Antibodies

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Complementary Products

Assay Kits

AB314826

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Secondary Antibodies

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Primary Antibodies

AB172730

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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