AB51870

Anti-ALK-1 antibody [MM0015-8G33]

Name: Anti-ALK-1 antibody [MM0015-8G33] (ab51870) | Abcam
Brand: Abcam Limited
SKU: AB51870
Price: 420 USD
Availability: InStock

(1 Review)

(4 Publications)

Mouse Monoclonal ALK-1 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ACVRL1.

View Alternative Names

ACVRLK1, ALK1, ACVRL1, Activin receptor type-1-like, Activin receptor-like kinase 1, Serine/threonine-protein kinase receptor R3, TGF-B superfamily receptor type I, ALK-1, SKR3, TSR-I

3 Images

Flow Cytometry - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)

Flow Cyt

Lab

Flow Cytometry - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)

Overlay histogram showing JEG3 cells stained with ab51870 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51870, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in JEG3 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)

ab51870 at 1/50 dilution staining ALK-1 in Human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Supplier Data

Western blot - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)

All lanes:

Western blot - Anti-ALK-1 antibody [MM0015-8G33] (ab51870) at 1/200 dilution

All lanes:

Human placenta tissue lysate

Predicted band size: 56 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0015-8G33

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ACVRL1.

P37023

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/800", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALK-1 also known as ACVRL1 or ALK1 protein is a receptor serine/threonine kinase with a molecular mass of approximately 55 kDa. It plays an important role in the TGF-beta superfamily signaling particularly in angiogenesis and blood vessel development. Expressed mainly in endothelial cells ALK-1 mediates cellular responses essential for maintaining vascular stability. The careful balance of its signaling is necessary indicating its importance in physiological conditions.

Biological function summary

The function of ALK-1 extends to the formation and maintenance of vascular endothelium as part of the larger receptor complex. It interacts with ligands such as BMP9 and BMP10 which regulate endothelial cell proliferation and differentiation. ALK-1's role as part of this receptor complex highlights its involvement in controlling the fine balance between angiogenic and anti-angiogenic processes. Its biological activity is vital for healthy vascular development and homeostasis.

Pathways

ALK-1 activation is a part of the BMP signaling pathway a branch of the larger TGF-beta pathway. This pathway is significant in various cellular processes including growth and differentiation. ALK-1 interacts with other signalling mediators like SMAD1/5/8 proteins which translocate to the nucleus to affect gene expression. Connecting with these pathways ALK-1 aligns closely with ACVR2A and ENG (Endoglin) which further highlight its role in vascular biology.

ALK-1 mutations or dysregulation leads to conditions such as hereditary hemorrhagic telangiectasia (HHT) and pulmonary arterial hypertension (PAH). These conditions showcase the important role ALK-1 plays in vascular integrity and signaling. ALK-1 is connected to disorders through interactions with proteins such as ENG further highlighting its involvement in vascular-related pathologies. Maintaining precise ALK-1 function is pivotal to preventing these disorders indicating its potential as a therapeutic target or biomarker.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.

See full target information ACVRL1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 7: PubMed35143420

2022

Cilia proteins are biomarkers of altered flow in the vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Ankan Gupta,Karthikeyan Thirugnanam,Madhan Thamilarasan,Ashraf M Mohieldin,Hadeel T Zedan,Shubhangi Prabhudesai,Meghan R Griffin,Andrew D Spearman,Amy Pan,Sean P Palecek,Huseyin C Yalcin,Surya M Nauli,Kevin R Rarick,Rahima Zennadi,Ramani Ramchandran

Cancers 13: PubMed34771445

2021

-2 Regulates Liver Fibrosis through the TGF-β Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ikuo Nakamura,Faizal Z Asumda,Catherine D Moser,Yoo Na N Kang,Jin-Ping Lai,Lewis R Roberts

Scientific reports 8:1561 PubMed29367682

2018

Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Lotta Tengroth,Julia Arebro,Olivia Larsson,Claus Bachert,Susanna Kumlien Georén,Lars-Olaf Cardell

Cell research 25:370-85 PubMed25656846

2015

EWI-2 negatively regulates TGF-β signaling leading to altered melanoma growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Xing Wang,Chandan Sharma,Konstantin Knoblich,Scott R Granter,Martin E Hemler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALK-1 antibody [MM0015-8G33]

Flow Cytometry - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)

Western blot - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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