Rabbit Recombinant Monoclonal ALK antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | IP | Flow Cyt | WB | IHC-P | IHC-Fr | |
---|---|---|---|---|---|---|
Human | Not recommended | Tested | Not recommended | Tested | Not recommended | Not recommended |
Mouse | Not recommended | Tested | Not recommended | Tested | Not recommended | Not recommended |
Rat | Not recommended | Expected | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system (PubMed:11121404, PubMed:11387242, PubMed:16317043, PubMed:17274988, PubMed:30061385, PubMed:34646012, PubMed:34819673). Also acts as a key thinness protein involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis (By similarity). Following activation by ALKAL2 ligand at the cell surface, transduces an extracellular signal into an intracellular response (PubMed:30061385, PubMed:33411331, PubMed:34646012, PubMed:34819673). In contrast, ALKAL1 is not a potent physiological ligand for ALK (PubMed:34646012). Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway (PubMed:34819673). Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif (PubMed:15226403, PubMed:16878150). Induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1 (PubMed:15226403, PubMed:16878150). ALK activation may also be regulated by pleiotrophin (PTN) and midkine (MDK) (PubMed:11278720, PubMed:11809760, PubMed:12107166, PubMed:12122009). PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation (PubMed:11278720, PubMed:11809760, PubMed:12107166). MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction (PubMed:12122009). Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase (PubMed:15226403, PubMed:16878150). Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK (PubMed:15226403, PubMed:16878150).
ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, ALK
Rabbit Recombinant Monoclonal ALK antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR24029-128
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ALK was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab275964 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275964 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug
Lane 2: ab275964 IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab275964 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (ab275964)
Predicted band size: 176 kDa
Observed band size: 250 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ALK undergoes glycosylation and phosphorylation. ALK has both extracellular and intracellular cleavage sites. The molecular weight observed are consistent with what have been described in literatures (PMID: 30541790, 32344689).
Negative control: kidney (PMID: 9053841).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-ALK antibody [EPR24029-128] (ab275964) at 1/1000 dilution
Lane 1: Mouse P5 brain tissue lysate at 40 µg
Lane 2: Mouse kidney tissue lysate at 40 µg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg
Lane 4: Rat P0 brain tissue lysate at 40 µg
Lane 5: Rat kidney tissue lysate at 40 µg
All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 176 kDa
Observed band size: 140-250 kDa
ALK was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate with ab275964 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275964 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug
Lane 2: ab275964 IP in IMR-32 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab275964 in IMR-32 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 119 seconds.
All lanes: Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (ab275964)
Predicted band size: 176 kDa
Observed band size: 140-250 kDa, 70 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ALK undergoes glycosylation and phosphorylation. ALK has both extracellular and intracellular cleavage sites. The 140-250 kDa bands observed are consistent with what have been described in literatures (PMID: 30541790, 32344689).
A 70-kDa band is observed, it may correspond to N terminal truncated ALK (PMID: 30400214).
Negative control: Raji and Ramos (PMID: 30541790).
Exposure time: 26 seconds.
All lanes: Western blot - Anti-ALK antibody [EPR24029-128] (ab275964) at 1/1000 dilution
Lane 1: SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 40 µg
Lane 2: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 40 µg
Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg
Lane 4: Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 40 µg
Lane 5: Ramos (human burkitts lymphoma b lymphocyte) whole cell lysate at 40 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 176 kDa
Observed band size: 140-250 kDa, 70 kDa
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