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Rabbit Recombinant Monoclonal ALK antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.

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Images

Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (AB275964), expandable thumbnail
  • Western blot - Anti-ALK antibody [EPR24029-128] (AB275964), expandable thumbnail
  • Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (AB275964), expandable thumbnail
  • Western blot - Anti-ALK antibody [EPR24029-128] (AB275964), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-PIHC-Fr
Human
Not recommended
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Tested
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Expected
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system (PubMed:11121404, PubMed:11387242, PubMed:16317043, PubMed:17274988, PubMed:30061385, PubMed:34646012, PubMed:34819673). Also acts as a key thinness protein involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis (By similarity). Following activation by ALKAL2 ligand at the cell surface, transduces an extracellular signal into an intracellular response (PubMed:30061385, PubMed:33411331, PubMed:34646012, PubMed:34819673). In contrast, ALKAL1 is not a potent physiological ligand for ALK (PubMed:34646012). Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway (PubMed:34819673). Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif (PubMed:15226403, PubMed:16878150). Induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1 (PubMed:15226403, PubMed:16878150). ALK activation may also be regulated by pleiotrophin (PTN) and midkine (MDK) (PubMed:11278720, PubMed:11809760, PubMed:12107166, PubMed:12122009). PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation (PubMed:11278720, PubMed:11809760, PubMed:12107166). MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction (PubMed:12122009). Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase (PubMed:15226403, PubMed:16878150). Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK (PubMed:15226403, PubMed:16878150).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ALK antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24029-128

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (ab275964), expandable thumbnail

    Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (ab275964)

    ALK was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab275964 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275964 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: ab275964 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab275964 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (ab275964)

    Predicted band size: 176 kDa

    Observed band size: 250 kDa

  • Western blot - Anti-ALK antibody [EPR24029-128] (ab275964), expandable thumbnail

    Western blot - Anti-ALK antibody [EPR24029-128] (ab275964)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ALK undergoes glycosylation and phosphorylation. ALK has both extracellular and intracellular cleavage sites. The molecular weight observed are consistent with what have been described in literatures (PMID: 30541790, 32344689).

    Negative control: kidney (PMID: 9053841).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-ALK antibody [EPR24029-128] (ab275964) at 1/1000 dilution

    Lane 1: Mouse P5 brain tissue lysate at 40 µg

    Lane 2: Mouse kidney tissue lysate at 40 µg

    Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 4: Rat P0 brain tissue lysate at 40 µg

    Lane 5: Rat kidney tissue lysate at 40 µg

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 176 kDa

    Observed band size: 140-250 kDa

  • Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (ab275964), expandable thumbnail

    Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (ab275964)

    ALK was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate with ab275964 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275964 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: ab275964 IP in IMR-32 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab275964 in IMR-32 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 119 seconds.

    All lanes: Immunoprecipitation - Anti-ALK antibody [EPR24029-128] (ab275964)

    Predicted band size: 176 kDa

    Observed band size: 140-250 kDa, 70 kDa

  • Western blot - Anti-ALK antibody [EPR24029-128] (ab275964), expandable thumbnail

    Western blot - Anti-ALK antibody [EPR24029-128] (ab275964)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ALK undergoes glycosylation and phosphorylation. ALK has both extracellular and intracellular cleavage sites. The 140-250 kDa bands observed are consistent with what have been described in literatures (PMID: 30541790, 32344689).

    A 70-kDa band is observed, it may correspond to N terminal truncated ALK (PMID: 30400214).

    Negative control: Raji and Ramos (PMID: 30541790).

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-ALK antibody [EPR24029-128] (ab275964) at 1/1000 dilution

    Lane 1: SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 2: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

    Lane 4: Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 40 µg

    Lane 5: Ramos (human burkitts lymphoma b lymphocyte) whole cell lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 176 kDa

    Observed band size: 140-250 kDa, 70 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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