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Rabbit Recombinant Monoclonal ALK antibody. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK antibody [SP144] (AB183332), expandable thumbnail
  • Western blot - Anti-ALK antibody [SP144] (AB183332), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK antibody [SP144] (AB183332), expandable thumbnail
  • Western blot - Anti-ALK antibody [SP144] (AB183332), expandable thumbnail
  • Western blot - Anti-ALK antibody [SP144] (AB183332), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab183322 for 10 minutes at room temperature.

Associated Products

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Target data

Function

Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. Thinness gene involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis (By similarity).

Alternative names

ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, ALK

Recommended products

Rabbit Recombinant Monoclonal ALK antibody. Suitable for WB, IHC-P and reacts with Human samples.

Alternative names

ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, ALK

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP144

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Anaplastic Lymphoma Kinase commonly referred to as ALK is a receptor tyrosine kinase known for its role in cell signaling. It is sometimes called CD246. ALK has a molecular mass of approximately 180 kDa. This protein finds expression predominantly in the central and peripheral nervous system with prominence in neural tissue during development. The ALK protein belongs to the insulin receptor superfamily exhibiting kinase activity that promotes signal transduction processes associated with growth and differentiation.

Biological function summary

ALK influences cell growth survival and differentiation playing a significant role during the development of the nervous system. As part of its biological activity the ALK protein can become a component of larger signaling complexes participating as an important activator or mediator. Evidence suggests ALK's involvement in neuronal differentiation and synaptogenesis. Its activity impacts intracellular pathways consequently modulating biological processes relevant to neural tissue and oncogenesis.

Pathways

ALK acts within the MAPK and PI3K-AKT signaling pathways. These pathways enable the transduction of signals from the cell surface to the nucleus influencing cellular outcomes such as proliferation and survival. In these pathways ALK interacts with various other proteins including GRB2 and PI3K which further facilitate downstream signaling. Proper functioning of these pathways is essential to maintaining cellular homeostasis and development.

Associated diseases and disorders

ALK has a significant relationship with certain types of cancer such as non-small cell lung carcinoma and anaplastic large cell lymphoma. Genetic alterations in ALK such as translocations or mutations can lead to the uncontrolled activation of its kinase activity resulting in oncogenic transformation. In these contexts the ALK protein often interacts with EML4 in lung cancer through a fusion forming an oncogenic driver. Targeting ALK with anti-ALK antibodies or small molecule inhibitors has emerged as a therapeutic strategy to manage these malignancies.

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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK antibody [SP144] (ab183332), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK antibody [SP144] (ab183332)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for ALK using ab183332 at 1/200 dilution in immunohistochemical analysis.

  • Western blot - Anti-ALK antibody [SP144] (ab183332), expandable thumbnail

    Western blot - Anti-ALK antibody [SP144] (ab183332)

    All lanes: Western blot - Anti-ALK antibody [SP144] (ab183332) at 1/200 dilution

    All lanes: Karpas whole cell lysate

    Predicted band size: 176 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK antibody [SP144] (ab183332), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK antibody [SP144] (ab183332)

    Formalin-fixed, paraffin-embedded human anaplastic lymphoma tissue stained for ALK using ab183332 at 1/200 dilution in immunohistochemical analysis.

  • Western blot - Anti-ALK antibody [SP144] (ab183332), expandable thumbnail

    Western blot - Anti-ALK antibody [SP144] (ab183332)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    KARPAS-299 cells carry the NPM-ALK fusion (PMID: 32193476, PMID: 9736036).

    Phosphorylated Tyr338/ Tyr567/ Tyr664 of NPM-ALK is equivalent to Tyr1278/ Tyr1507/ Tyr1604 of full length ALK (PMID: 19887368).

    Bands around 300 kDa may be due to protein aggregation (PMID: 9121481).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-ALK antibody [SP144] (ab183332) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] ab322000) at 1/1000 dilution

    Lane 1: Untreated KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate at 20 µg

    Lane 2: KARPAS-299 treated with 1 uM Crizotinib for 1 hour whole cell lysate, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 80 kDa, 300 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ALK antibody [SP144] (ab183332), expandable thumbnail

    Western blot - Anti-ALK antibody [SP144] (ab183332)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    KARPAS-299 cells carry the NPM-ALK fusion (PMID: 32193476, PMID: 9736036).

    Phosphorylated Tyr338/ Tyr567/ Tyr664 of NPM-ALK is equivalent to Tyr1278/ Tyr1507/ Tyr1604 of full length ALK (PMID: 19887368).

    Bands around 300 kDa may be due to protein aggregation (PMID: 9121481).

    Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-ALK antibody [SP144] (ab183332) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] ab322000) at 1/1000 dilution

    Lane 1: KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate (Untreated membrane) at 20 µg

    Lane 2: KARPAS-299 whole cell lysate (phosphatase treated membrane) at 20 µg

    Lane 3: KARPAS-299 treated with 50 uM pervanadate for 30 minutes, whole cell lysate (Untreated membrane) at 20 µg

    Lane 4: KARPAS-299 treated with 50 uM pervanadate for 30 minutes, whole cell lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 80 kDa, 300 kDa

    Exposure time: 59s

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com