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Rabbit Recombinant Multiclonal ALK phospho Y1278 + Y1507 + Y1604 antibody. Suitable for Dot, WB, IP and reacts with Synthetic peptide - Human, Human samples.

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Images

Dot Blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (AB322000), expandable thumbnail
  • Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (AB322000), expandable thumbnail
  • Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (AB322000), expandable thumbnail
  • Immunoprecipitation - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (AB322000), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBIPIHC-P
Human
Expected
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Synthetic peptide - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Target data

Function

Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. Thinness gene involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis (By similarity).

Targets

ALK phospho Y1278, ALK phospho Y1507, ALK

Alternative names

ALK tyrosine kinase receptor, Anaplastic lymphoma kinase

Recommended products

Rabbit Recombinant Multiclonal ALK phospho Y1278 + Y1507 + Y1604 antibody. Suitable for Dot, WB, IP and reacts with Synthetic peptide - Human, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1164

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Anaplastic Lymphoma Kinase commonly referred to as ALK is a receptor tyrosine kinase known for its role in cell signaling. It is sometimes called CD246. ALK has a molecular mass of approximately 180 kDa. This protein finds expression predominantly in the central and peripheral nervous system with prominence in neural tissue during development. The ALK protein belongs to the insulin receptor superfamily exhibiting kinase activity that promotes signal transduction processes associated with growth and differentiation.

Biological function summary

ALK influences cell growth survival and differentiation playing a significant role during the development of the nervous system. As part of its biological activity the ALK protein can become a component of larger signaling complexes participating as an important activator or mediator. Evidence suggests ALK's involvement in neuronal differentiation and synaptogenesis. Its activity impacts intracellular pathways consequently modulating biological processes relevant to neural tissue and oncogenesis.

Pathways

ALK acts within the MAPK and PI3K-AKT signaling pathways. These pathways enable the transduction of signals from the cell surface to the nucleus influencing cellular outcomes such as proliferation and survival. In these pathways ALK interacts with various other proteins including GRB2 and PI3K which further facilitate downstream signaling. Proper functioning of these pathways is essential to maintaining cellular homeostasis and development.

Associated diseases and disorders

ALK has a significant relationship with certain types of cancer such as non-small cell lung carcinoma and anaplastic large cell lymphoma. Genetic alterations in ALK such as translocations or mutations can lead to the uncontrolled activation of its kinase activity resulting in oncogenic transformation. In these contexts the ALK protein often interacts with EML4 in lung cancer through a fusion forming an oncogenic driver. Targeting ALK with anti-ALK antibodies or small molecule inhibitors has emerged as a therapeutic strategy to manage these malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Dot Blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000), expandable thumbnail

    Dot Blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000)

    Dot blot analysis of ALK (phospho Y1278 + Y1507 + Y1604) using ab322000 at 1:1000 dilution (0.476 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: Human ALK tyrosine kinase receptor (phospho Y1604) peptide (aa1600-1609)

    Lane2: Human ALK tyrosine kinase receptor peptide (aa1599-1610)

    Lane3: Human ALK tyrosine kinase receptor (phospho Y1507) peptide (aa1501-1516)

    Lane4: Human ALK tyrosine kinase receptor peptide (aa1500-1516)

    Lane5: Human ALK tyrosine kinase receptor (phospho Y1278) peptide (aa1272-1284)

    Lane6: Human ALK tyrosine kinase receptor peptide (aa1272-1284)

    Lane7: Human ALK tyrosine kinase receptor (phospho Y1282) peptide (aa1272-1286)

    Lane8: Human ALK tyrosine kinase receptor (phospho Y1283) peptide (aa1272-1286)

    Lane9: Human ALK tyrosine kinase receptor (phospho Y1282+Y1283) peptide (aa1272-1286)

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Dot Blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000) at 1/1000 dilution

    Lane 1: Human ALK tyrosine kinase receptor (phospho Y1604) peptide (aa1600-1609)

    Lane 2: Human ALK tyrosine kinase receptor peptide (aa1599-1610)

    Lane 3: Human ALK tyrosine kinase receptor (phospho Y1507) peptide (aa1501-1516)

    Lane 4: Human ALK tyrosine kinase receptor peptide (aa1500-1516)

    Lane 5: Human ALK tyrosine kinase receptor (phospho Y1278) peptide (aa1272-1284)

    Lane 6: Human ALK tyrosine kinase receptor peptide (aa1272-1284)

    Lane 7: Human ALK tyrosine kinase receptor (phospho Y1282) peptide (aa1272-1286)

    Lane 8: Human ALK tyrosine kinase receptor (phospho Y1283) peptide (aa1272-1286)

    Lane 9: Human ALK tyrosine kinase receptor (phospho Y1282+Y1283) peptide (aa1272-1286)

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000), expandable thumbnail

    Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    KARPAS-299 cells carry the NPM-ALK fusion (PMID: 32193476, PMID: 9736036).

    Phosphorylated Tyr338/ Tyr567/ Tyr664 of NPM-ALK is equivalent to Tyr1278/ Tyr1507/ Tyr1604 of full length ALK (PMID: 19887368).

    Bands around 300 kDa may be due to protein aggregation (PMID: 9121481).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-ALK antibody [SP144] (Anti-ALK antibody [SP144] ab183332) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000) at 1/1000 dilution

    Lane 1: Untreated KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate at 20 µg

    Lane 2: KARPAS-299 treated with 1 uM Crizotinib for 1 hour whole cell lysate, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 80 kDa, 300 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000), expandable thumbnail

    Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    KARPAS-299 cells carry the NPM-ALK fusion (PMID: 32193476, PMID: 9736036).

    Phosphorylated Tyr338/ Tyr567/ Tyr664 of NPM-ALK is equivalent to Tyr1278/ Tyr1507/ Tyr1604 of full length ALK (PMID: 19887368).

    Bands around 300 kDa may be due to protein aggregation (PMID: 9121481).

    Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-ALK antibody [SP144] (Anti-ALK antibody [SP144] ab183332) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000) at 1/1000 dilution

    Lane 1: KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate (Untreated membrane) at 20 µg

    Lane 2: KARPAS-299 whole cell lysate (phosphatase treated membrane) at 20 µg

    Lane 3: KARPAS-299 treated with 50 uM pervanadate for 30 minutes, whole cell lysate (Untreated membrane) at 20 µg

    Lane 4: KARPAS-299 treated with 50 uM pervanadate for 30 minutes, whole cell lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 80 kDa, 300 kDa

    Exposure time: 59s

  • Immunoprecipitation - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000), expandable thumbnail

    Immunoprecipitation - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000)

    ALK (phospho Y1278 + Y1507 + Y1604) was immunoprecipitated from 0.35 mg KARPAS-299 (human T cell lymphoma cell) whole cell lysate with ab322000 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322000 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: KARPAS-299 (human T cell lymphoma cell) whole cell lysate

    Lane 2: ab322000 IP in KARPAS-299 (human T cell lymphoma cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab322000 in KARPAS-299 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-ALK (phospho Y1278 + Y1507 + Y1604) antibody [RM1164] (ab322000) at 1/30 dilution

    All lanes: KARPAS-299 (human T cell lymphoma cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

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Product protocols

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