AB49746

Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1]

Name: Alkaline Phosphatase conjugated Anti-6X His tag® antibody [HIS-1] (ab49746) | Abcam
Brand: Abcam Limited
SKU: AB49746
Rating: 3 (1 reviews)

(1 Review)

(8 Publications)

Anti-6X His tag® antibody [HIS-1] - Alkaline Phosphatase conjugated (ab49746) is a mouse monoclonal antibody detecting 6X His tag® in Western Blot, ELISA, Dot Blot.

- Trusted since 2007

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

Unconjugated

Anti-6X His tag® antibody [HIS-1]
HRP

HRP Anti-polyHistidine antibody [HIS-1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIS-1

Isotype

IgG2a

Conjugation

Alkaline Phosphatase

Excitation/Emission

Carrier free

Applications

WB, ELISA, Dot

applications

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Reactivity data

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Product details

What is this antibody validated in?
Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1] (ab49746) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), ELISA, Dot Blot. Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

What is the molecular weight of 6X His tag®?
Anti-6X His tag® [HIS-1] Alkaline Phosphatase (ab49746) specifically detects a band for 6X His tag® (UniProt: P26367) at a molecular weight of 7kDa.

Related products
Antibody clone HIS-1 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® Alkaline Phosphatase [HIS-1] (ab49746).

Other related products
We have a range of other formats of antibody clone [HIS-1] also available for your convenience: ab213204, Carrier free - ab232492, Alexa Fluor® 488 - ab237336, Alexa Fluor® 647 - ab237337, PE - ab237338, HRP - ab237339, Biotin - ab316173, APC - ab317659, FITC - ab318311, Alkaline Phosphatase - ab49746

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 8 Preservative: 0.097% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.06% Tris buffered saline, 0.0095% Magnesium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3999 PubMed40312392

2025

An intranasal subunit vaccine induces protective systemic and mucosal antibody immunity against respiratory viruses in mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Aina Karen Anthi,Anette Kolderup,Eline Benno Vaage,Malin Bern,Sopisa Benjakul,Elias Tjärnhage,Fulgencio Ruso-Julve,Kjell-Rune Jensen,Heidrun Elisabeth Lode,Marina Vaysburd,Jeannette Nilsen,Marie Leangen Herigstad,Siri Aastedatter Sakya,Lisa Tietze,Diego Pilati,Mari Nyquist-Andersen,Mirjam Dürkoop,Torleif Tollefsrud Gjølberg,Linghang Peng,Stian Foss,Morten C Moe,Benjamin E Low,Michael V Wiles,David Nemazee,Frode L Jahnsen,John Torgils Vaage,Kenneth A Howard,Inger Sandlie,Leo C James,Gunnveig Grødeland,Fridtjof Lund-Johansen,Jan Terje Andersen

PNAS nexus 4:pgaf042 PubMed40041621

2025

Engineering of anticancer human immunoglobulin A equipped with albumin for enhanced plasma half-life.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Mester,Chilam Chan,Marta Lustig,Stian Foss,J H Marco Jansen,Marie Leangen Herigstad,Mitchell Evers,Jeannette Nilsen,Karli R Reiding,J Mirjam A Damen,Renate Burger,Algirdas Grevys,Bjørn Dalhus,Thomas Valerius,Inger Sandlie,Jeanette H W Leusen,Jan Terje Andersen

Cells 12: PubMed37566065

2023

DNA-Binding Activity of CAMTA3 Is Essential for Its Function: Identification of Critical Amino Acids for Its Transcriptional Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Kasavajhala V S K Prasad,Amira A E Abdel-Hameed,Qiyan Jiang,Anireddy S N Reddy

PeerJ 9:e12380 PubMed34966572

2021

Overexpression of fucosyltransferase 8 reverses the inhibitory effect of high-dose dexamethasone on osteogenic response of MC3T3-E1 preosteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiming Wu,Tianye Lin,Pan Kang,Zhikun Zhuang,Haibin Wang,Wei He,Qiushi Wei,Ziqi Li

The FEBS journal 287:3827-3840 PubMed32153092

2020

The copper-responsive ScsC protein of Salmonella promotes intramacrophage survival and interacts with the arginine sensor ArtI.

Applications

Unspecified application

Species

Unspecified reactive species

Buke Yucel,Gary K Robinson,Mark Shepherd

Cell host & microbe 25:617-629.e7 PubMed30926239

2019

Complement C4 Prevents Viral Infection through Capsid Inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Bottermann,Stian Foss,Sarah L Caddy,Dean Clift,Laurens M van Tienen,Marina Vaysburd,James Cruickshank,Kevin O'Connell,Jessica Clark,Keith Mayes,Katie Higginson,Heidrun E Lode,Martin B McAdam,Inger Sandlie,Jan Terje Andersen,Leo C James

Extremophiles : life under extreme conditions 18:905-13 PubMed25102813

2014

Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD.

Applications

Unspecified application

Species

Unspecified reactive species

Anna-Lena Henche,Marleen van Wolferen,Abhrajyoti Ghosh,Sonja-Verena Albers

The Journal of biological chemistry 286:8961-76 PubMed21216961

2011

Neutralization of Clostridium difficile toxin A with single-domain antibodies targeting the cell receptor binding domain.

Applications

Species

Unspecified reactive species

Greg Hussack,Mehdi Arbabi-Ghahroudi,Henk van Faassen,J Glenn Songer,Kenneth K-S Ng,Roger MacKenzie,Jamshid Tanha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alkaline Phosphatase Anti-6X His tag® antibody [HIS-1]

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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