AB354

Anti-Alkaline Phosphatase antibody

Name: Anti-Alkaline Phosphatase antibody (ab354) | Abcam
Brand: Abcam Limited
SKU: AB354
Rating: 5 (2 reviews)

(2 Reviews)

(13 Publications)

Rabbit Polyclonal PPB antibody. Suitable for IP, ELISA, WB and reacts with Escherichia coli samples. Cited in 13 publications. Immunogen corresponding to Full Length Protein corresponding to Escherichia coli K-12 phoA.

View Alternative Names

b0383, JW0374, phoA, Alkaline phosphatase, APase

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Escherichia coli

Applications

WB, IP, ELISA

applications

Immunogen

Full Length Protein corresponding to Escherichia coli K-12 phoA. The exact immunogen used to generate this antibody is proprietary information.

P00634

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Purification notes

This product was prepared from monospecific antiserum by a delipidation and defibrination.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alkaline Phosphatase also known as ALP or ALKPHOS is an enzyme that removes phosphate groups from various molecules like nucleotides and proteins. This enzymatic activity requires alkaline conditions with a pH optimum around 8–10. ALP is anchored to the cell membrane and exists in several isoforms each with a mass around 57-70 kDa. Different tissues express specific isoforms such as bone liver kidney and placenta. Osteoblasts for example show high levels of alkaline phosphatase expression often detected by techniques like ALP staining or alkaline phosphatase staining.

Biological function summary

Alkaline phosphatase plays important roles in dephosphorylation processes. Particularly in bone it is associated with osteoblast activity where it is important for bone mineralization. The enzyme is not known to be part of a large protein complex but its activity aids in the conversion of inorganic pyrophosphate into phosphate which promotes the deposition of calcium phosphate in bone. ALP staining and ALP staining osteoblast can be used in laboratory settings for studying the mineralization and function of osteoblasts.

Pathways

Alkaline phosphatase is key in the regulation of the phosphate group levels within the organism particularly in the bone mineralization process. It participates in the vitamin D metabolic pathway which influences calcium and phosphorus homeostasis. Its function has a dynamic relationship with proteins like NPP1 which regulates levels of inorganic pyrophosphate acting counter to alkaline phosphatase's phosphate-producing activity.

Defects in alkaline phosphatase activity are linked to conditions like hypophosphatasia and hyperphosphatasia where mineralization processes are disrupted. In hypophosphatasia decreased ALP activity leads to improper bone formation due to accumulation of inorganic pyrophosphate. This interaction is notable alongside proteins such as tissular nonspecific alkaline phosphatase. On the other hand elevated ALP levels often indicate liver or bone disease pathology where increased enzyme levels reflect altered tissue turnover.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information phoA

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 43:962-975 PubMed38355807

2024

Targeted inhibition of SCF confers anti-tumor activities resulting in a survival benefit in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jichuan Wang,Alexander Ferrena,Ranxin Zhang,Swapnil Singh,Valentina Viscarret,Waleed Al-Harden,Osama Aldahamsheh,Hasibagan Borjihan,Amit Singla,Simon Yaguare,Janet Tingling,Xiaolin Zi,Yungtai Lo,Richard Gorlick,Edward L Schwartz,Hongling Zhao,Rui Yang,David S Geller,Deyou Zheng,Bang H Hoang

Nature methods 21:342-352 PubMed38191931

2024

Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjian He,Jiaze Yin,Mingsheng Li,Chinmayee Vallabh Prabhu Dessai,Meihui Yi,Xinyan Teng,Meng Zhang,Yueming Li,Zhiyi Du,Bing Xu,Ji-Xin Cheng

International journal of molecular sciences 24: PubMed36769191

2023

Regulatory Mechanism between Ferritin and Mitochondrial Reactive Oxygen Species in Spinal Ligament-Derived Cells from Ossification of Posterior Longitudinal Ligament Patient.

Applications

Unspecified application

Species

Unspecified reactive species

Jong Tae Kim,Yonggoo Kim,Ji Yeon Kim,Seungok Lee,Myungshin Kim,Dong Wook Jekarl

RSC advances 12:5557-5570 PubMed35425568

2022

Reduced graphene oxide-incorporated calcium phosphate cements with pulsed electromagnetic fields for bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hoon Seonwoo,Han-Wool Choung,Sangbae Park,Kyoung Soon Choi,Kyoung-Je Jang,Jangho Kim,Ki-Taek Lim,Yeonju Kim,Pankaj Garg,Shambhavi Pandey,Juo Lee,Joo-Cheol Park,Yun-Hoon Choung,Pill-Hoon Choung,Soo Young Kim,Jong Hoon Chung

Reports of biochemistry & molecular biology 9:331-337 PubMed33649727

2021

Comparison of Osteogenic Potential of Phenytoin with Dexamethasone in Cultured Dental Pulp Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mitra Asgharian-Rezaee,Raheleh Alipour-Farmad,Zahra Tayarani-Najaran

Science advances 7: PubMed33637520

2021

The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Hodgkinson,P Monica Tsimbouri,Virginia Llopis-Hernandez,Paul Campsie,David Scurr,Peter G Childs,David Phillips,Sam Donnelly,Julia A Wells,Fergal J O'Brien,Manuel Salmeron-Sanchez,Karl Burgess,Morgan Alexander,Massimo Vassalli,Richard O C Oreffo,Stuart Reid,David J France,Matthew J Dalby

Annals of the New York Academy of Sciences 1490:90-104 PubMed33594717

2021

The interaction of SKP2 with p27 enhances the progression and stemness of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jichuan Wang,Osama Aldahamsheh,Alexander Ferrena,Hasibagan Borjihan,Amit Singla,Simon Yaguare,Swapnil Singh,Valentina Viscarret,Janet Tingling,Xiaolin Zi,Yungtai Lo,Richard Gorlick,Deyou Zheng,Edward L Schwartz,Hongling Zhao,Rui Yang,David S Geller,Bang H Hoang

American journal of physiology. Lung cellular and molecular physiology 318:L429-L441 PubMed31850803

2019

dysfunction impairs insulin signaling and glucose homeostasis in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Anna R Hemnes,Joshua P Fessel,Xinping Chen,Shijun Zhu,Niki L Fortune,Christopher Jetter,Michael Freeman,John H Newman,James D West,Megha H Talati

Journal of natural medicines 72:929-936 PubMed30043216

2018

Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chenguang Wang,Xin Wan,Yun Li,Huafeng Zhang,Lei Zhang

Nature biomedical engineering 1:758-770 PubMed31015671

2017

Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor.

Applications

Unspecified application

Species

Unspecified reactive species

Penelope M Tsimbouri,Peter G Childs,Gabriel D Pemberton,Jingli Yang,Vineetha Jayawarna,Wich Orapiriyakul,Karl Burgess,Cristina González-García,Gavin Blackburn,Dilip Thomas,Catalina Vallejo-Giraldo,Manus J P Biggs,Adam S G Curtis,Manuel Salmerón-Sánchez,Stuart Reid,Matthew J Dalby

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Alkaline Phosphatase antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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