AB308353

Anti-Alkaline phosphatase antibody [HL1267]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PPBI antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Transfected cell lysate samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALPI.

View Alternative Names

Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI

5 Images

Immunohistochemistry paraffin embedded sections - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Immunohistochemical analysis of mouse intestine tissue labeling Alkaline phosphatase using ab308353 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Non-transfected (–) and transfected (+) HEK293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase antibody [HL1267] (ab308353) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (ab308353) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK293T whole cell lysate at 30 µg

Lanes 2 - 4:

Transfected (+) HEK293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56.8 kDa

false

Supplier Data

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (ab308353) at 1/1000 dilution

Lane 1:

Caco-2 cell lysate at 30 µg

Lane 2:

HCT116 cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase antibody [HL1267] (ab308353) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (ab308353) at 1/1000 dilution

Lane 1:

Mouse small intestine tissue lysate at 50 µg

Lane 2:

Mouse spleen tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56.8 kDa

false

Supplier Data

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1267] (ab308353) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

All lanes:

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (ab308353) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 50 µg

Lane 2:

Mouse ovary tissue lysate at 50 µg

Lane 3:

Mouse intestine tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56.8 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1267

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ALPI. The exact immunogen used to generate this antibody is proprietary information.

P09923

Specificity

This antibody is specific for human ALPI, ALPP, and ALPG protein, and it does not cross-react with human ALPL protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alkaline Phosphatase also known as ALP or ALKPHOS is an enzyme that removes phosphate groups from various molecules like nucleotides and proteins. This enzymatic activity requires alkaline conditions with a pH optimum around 8–10. ALP is anchored to the cell membrane and exists in several isoforms each with a mass around 57-70 kDa. Different tissues express specific isoforms such as bone liver kidney and placenta. Osteoblasts for example show high levels of alkaline phosphatase expression often detected by techniques like ALP staining or alkaline phosphatase staining.

Biological function summary

Alkaline phosphatase plays important roles in dephosphorylation processes. Particularly in bone it is associated with osteoblast activity where it is important for bone mineralization. The enzyme is not known to be part of a large protein complex but its activity aids in the conversion of inorganic pyrophosphate into phosphate which promotes the deposition of calcium phosphate in bone. ALP staining and ALP staining osteoblast can be used in laboratory settings for studying the mineralization and function of osteoblasts.

Pathways

Alkaline phosphatase is key in the regulation of the phosphate group levels within the organism particularly in the bone mineralization process. It participates in the vitamin D metabolic pathway which influences calcium and phosphorus homeostasis. Its function has a dynamic relationship with proteins like NPP1 which regulates levels of inorganic pyrophosphate acting counter to alkaline phosphatase's phosphate-producing activity.

Defects in alkaline phosphatase activity are linked to conditions like hypophosphatasia and hyperphosphatasia where mineralization processes are disrupted. In hypophosphatasia decreased ALP activity leads to improper bone formation due to accumulation of inorganic pyrophosphate. This interaction is notable alongside proteins such as tissular nonspecific alkaline phosphatase. On the other hand elevated ALP levels often indicate liver or bone disease pathology where increased enzyme levels reflect altered tissue turnover.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPI

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 42:1933-1942 PubMed38520666

2024

Cardamonin inhibits osteogenic differentiation by downregulating Wnt/beta-catenin signaling and alleviates subchondral osteosclerosis in osteoarthritic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fanding Meng,Pengchong Zhu,Xiaoli Ren,Limei Wang,Dong Ding,Jiangbo Yan,Ying Zhang,Shang-You Yang,Bin Ning

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alkaline phosphatase antibody [HL1267]

Immunohistochemistry paraffin embedded sections - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Western blot - Anti-Alkaline phosphatase antibody [HL1267] (AB308353)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise