Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Alkaline Phosphatase. Suitable for WB and reacts with Tag samples.
Images
![Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB81655), expandable thumbnail](https://content.abcam.com/products/images/alkaline-phosphatase-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab81655--western-blot-img76793.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB81655)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Alkaline Phosphatase
- Storage buffer
pH: 7.2
Preservative: 0.013% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3025% Tris- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | |
|---|---|
| Tag | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/5000 | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
8 products for Alternative Version
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Alkaline Phosphatase. Suitable for WB and reacts with Tag samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Alkaline Phosphatase
- Storage buffer
pH: 7.2
Preservative: 0.013% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3025% Tris- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- M2
- Specificity
ab81655 recognizes the DYKDDDDK tag sequence (also known as the FLAG® tag sequence) at an N-terminal, Met-N-terminal, C-terminal, or internal site.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655), expandable thumbnail](https://content.abcam.com/products/images/alkaline-phosphatase-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab81655--western-blot-img76793.jpg "Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)")
Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
Imaged using stabilized Alkaline Phosphatase substrate.
All lanes: Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655) at 1/10000 dilution
Lane 1: 60 kDa DYKDDDDK-tagged protein at 0.3 µg
Lane 2: 60 kDa DYKDDDDK-tagged protein at 0.01 µg
Lane 3: 60 kDa DYKDDDDK-tagged protein at 0.03 µg
Lane 4: 60 kDa DYKDDDDK-tagged protein at 0.001 µg
Lane 5: 60 kDa DYKDDDDK-tagged protein at 0.003 µg
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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