AB81655

Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Name: Anti-DDDDK tag (Binds to FLAG® tag sequence) Alkaline Phosphatase antibody [M2] (ab81655) | Abcam
Brand: Abcam Limited
SKU: AB81655

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(1 Publication)

Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Alkaline Phosphatase. Suitable for WB and reacts with Tag samples. Cited in 1 publication.

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

1 Images

Supplier Data

Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB81655)

Imaged using stabilized Alkaline Phosphatase substrate.

All lanes:

Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655) at 1/10000 dilution

Lane 1:

60 kDa DYKDDDDK-tagged protein at 0.3 µg

Lane 2:

60 kDa DYKDDDDK-tagged protein at 0.01 µg

Lane 3:

60 kDa DYKDDDDK-tagged protein at 0.03 µg

Lane 4:

60 kDa DYKDDDDK-tagged protein at 0.001 µg

Lane 5:

60 kDa DYKDDDDK-tagged protein at 0.003 µg

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Conjugation

Alkaline Phosphatase

Excitation/Emission

Carrier free

Applications

applications

Specificity

ab81655 recognizes the DYKDDDDK tag sequence (also known as the FLAG® tag sequence) at an N-terminal, Met-N-terminal, C-terminal, or internal site.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 Preservative: 0.013% Sodium azide Constituents: 0.87% Sodium chloride, 0.3025% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 5:3610-6 PubMed3915782

1985

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

Applications

Unspecified application

Species

Unspecified reactive species

G I Evan,G K Lewis,G Ramsay,J M Bishop

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Western blot - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB81655)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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