AB6661

Alkaline Phosphatase Anti-GFP antibody

Name: Anti-GFP Alkaline Phosphatase antibody (ab6661) Goat polyclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB6661
Price: 535 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal GFP antibody - conjugated to Alkaline Phosphatase. Suitable for ELISA, Dot, WB.

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

2 Images

Supplier Data

Western blot - Alkaline Phosphatase Anti-GFP antibody (AB6661)

All lanes:

Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661) at 1/1000 dilution

All lanes:

GFP at 0.05 µg

Predicted band size: 27 kDa

false

Supplier Data

Dot Blot - Alkaline Phosphatase Anti-GFP antibody (AB6661)

Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.

Lane 1 : 100 ng GFP protein
Lane 2 : 33.33 ng GFP protein
Lane 3 : 11.11 ng GFP protein
Lane 4 : 3.70 ng GFP protein
Lane 5 : 1.23 ng GFP protein

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Alkaline Phosphatase

Excitation/Emission

Carrier free

Applications

Dot, ELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Aequorea victoria GFP.

P42212

Specificity

Antibody recognizes wild type, recombinant and enhanced forms of GFP. Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. No reaction was observed against human, mouse and rat serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "This product has been assayed against 1.0 μg of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.

Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 8 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

See full target information GFP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Applied microbiology and biotechnology 106:1677-1689 PubMed35129657

2022

Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley E Sproles,Anthony Berndt,Francis J Fields,Stephen P Mayfield

PloS one 16:e0257089 PubMed34793485

2021

Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony J Berndt,Tressa N Smalley,Bijie Ren,Ryan Simkovsky,Amr Badary,Ashley E Sproles,Francis J Fields,Yasin Torres-Tiji,Vanessa Heredia,Stephen P Mayfield

PloS one 8:e59435 PubMed23555033

2013

Fission yeast CSL proteins function as transcription factors.

Applications

Species

Unspecified reactive species

Martina Oravcová,Mikoláš Teska,František Půta,Petr Folk,Martin Převorovský

View all publications

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