Goat Polyclonal GFP antibody - conjugated to Alkaline Phosphatase. Suitable for ELISA, Dot, WB and reacts with Tag samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Aequorea victoria GFP.
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Publications
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- Applied microbiology and biotechnology 106:1677-16892022Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshley E Sproles,Anthony Berndt,Francis J Fields,Stephen P MayfieldPubMed 35129657
- PloS one 16:e02570892021Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnthony J Berndt,Tressa N Smalley,Bijie Ren,Ryan Simkovsky,Amr Badary,Ashley E Sproles,Francis J Fields,Yasin Torres-Tiji,Vanessa Heredia,Stephen P MayfieldPubMed 34793485
- PloS one 8:e594352013Fission yeast CSL proteins function as transcription factors.Applications:WBReactive species:Unspecified reactive speciesMartina Oravcová,Mikoláš Teska,František Půta,Petr Folk,Martin PřevorovskýPubMed 23555033
Key facts
- Isotype
- IgG
- Host species
- Goat
- Conjugation
- Alkaline Phosphatase
- Storage buffer
pH: 8
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Aequorea victoria GFP. Database link P42212
Reactivity data
Select an application| ELISA | Dot | WB | |
|---|---|---|---|
| Tag | Expected | Tested | Tested |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes This product has been assayed against 1.0 μg of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/1000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
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Target data
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Alternative names
Green fluorescent protein, GFP
Recommended products
Goat Polyclonal GFP antibody - conjugated to Alkaline Phosphatase. Suitable for ELISA, Dot, WB and reacts with Tag samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Aequorea victoria GFP.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Conjugation
- Alkaline Phosphatase
- Storage buffer
pH: 8
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Aequorea victoria GFP. Database link P42212
- Purification technique
- Affinity purification
- Specificity
Antibody recognizes wild type, recombinant and enhanced forms of GFP. Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. No reaction was observed against human, mouse and rat serum proteins.
- Concentration
- Purification notes
Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)
All lanes: Western blot - Alkaline Phosphatase Anti-GFP antibody (ab6661) at 1/1000 dilution
All lanes: GFP at 0.05 µg with Blocking buffer
Predicted band size: 27 kDa
Dot Blot - Alkaline Phosphatase Anti-GFP antibody (ab6661)
Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.
Lane 1: 100 ng GFP protein
Lane 2: 33.33 ng GFP protein
Lane 3: 11.11 ng GFP protein
Lane 4: 3.70 ng GFP protein
Lane 5: 1.23 ng GFP protein
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