AB81656

Alkaline Phosphatase Anti-HA tag antibody [16B12]

Name: Anti-HA tag Alkaline Phosphatase antibody [16B12] (ab81656) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB81656
Price: 520 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal HA tag antibody - conjugated to Alkaline Phosphatase. Suitable for WB and reacts with Tag samples. Cited in 2 publications.

View Alternative Names

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

1 Images

Supplier Data

Western blot - Alkaline Phosphatase Anti-HA tag antibody [16B12] (AB81656)

Imaged using stabilized Alkaline Phosphatase substrate.

All lanes:

Western blot - Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656) at 1/10000 dilution

Lane 1:

60 kDa HA-tagged protein at 0.3 µg

Lane 2:

60 kDa HA-tagged protein at 0.01 µg

Lane 3:

60 kDa HA-tagged protein at 0.03 µg

Lane 4:

60 kDa HA-tagged protein at 0.001 µg

Lane 5:

60 kDa HA-tagged protein at 0.003 µg

Predicted band size: 64 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

16B12

Isotype

IgG1

Conjugation

Alkaline Phosphatase

Excitation/Emission

Carrier free

Applications

applications

Epitope

The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.2 Preservative: 0.013% Sodium azide Constituents: 0.87% Sodium chloride, 0.3025% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information HA tag

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The New phytologist 240:1202-1218 PubMed37559429

2023

A prophage tail-like protein facilitates the endophytic growth of Burkholderia gladioli and mounting immunity in tomato.

Applications

Unspecified application

Species

Unspecified reactive species

Ravindra Kumar Chandan,Rahul Kumar,Kristi Kabyashree,Sunil Kumar Yadav,Mandira Roy,Durga Madhab Swain,Gopaljee Jha

Molecular and cellular biology 5:3610-6 PubMed3915782

1985

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

Applications

Unspecified application

Species

Unspecified reactive species

G I Evan,G K Lewis,G Ramsay,J M Bishop

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alkaline Phosphatase Anti-HA tag antibody [16B12]

Western blot - Alkaline Phosphatase Anti-HA tag antibody [16B12] (AB81656)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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