AB307798

Anti-Alkaline phosphatase (intestinal) antibody [HL1268]

Recombinant
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Rabbit Recombinant Monoclonal PPBI antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Drosophila melanogaster, Transfected cell line - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ALPI.

View Alternative Names

Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

Immunohistochemical analysis of paraffin embedded mouse colon tissue detecting Alkaline Phosphotase with ab307798 rabbit monoclonal anti-Alkaline phosphatase (intestinal) antibody [HL1268]. ab307798 was used at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

Various tissue extracts were separated by 7.5% SDS-PAGE

All lanes:

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (ab307798) at 1/1000 dilution

Lane 1:

Mouse testis tissue extract at 30 µg

Lane 2:

Mouse ovary tissue extract at 30 µg

Lane 3:

Mouse intestine tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

All lanes:

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (ab307798) at 1/1000 dilution

All lanes:

Whole S2 cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

Non-transfected (–) and transfected (+) 293T whole cell extracts (Alkaline phosphatase Isozymes) were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (ab307798) at 1/5000 dilution

Lane 1:

Non transfected (-) 293T whole cell extract without epitope tag at 30 µg

Lane 2:

ALPI transfected (+) 293T whole cell extract with DDDDK epitope tag at 30 µg

Lane 3:

ALPP transfected (+) 293T whole cell extract with DDDDK epitope tag at 30 µg

Lane 4:

ALPG transfected (+) 293T whole cell extract with DDDDK epitope tag at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1268

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human, Drosophila melanogaster

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ALPI. The exact immunogen used to generate this antibody is proprietary information.

P09923

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Intestinal alkaline phosphatase also known as alkaline phosphatase intestinal or intestinal ALP is an enzyme that plays an important mechanical role in dephosphorylation processes. This enzyme with a molecular weight (mw) of approximately 57 kDa is primarily expressed in the brush border of enterocytes lining the intestinal tract. It exhibits phosphatase activity by removing phosphate groups from various molecules including nucleotides proteins and alkaloids to regulate diverse physiological processes.

Biological function summary

Intestinal alkaline phosphatase is involved in maintaining gut health and homeostasis. It participates in the detoxification of lipopolysaccharides (LPS) and modulation of gut microbiota thereby reducing inflammation and protecting intestinal barrier function. This enzyme does not form part of a complex; instead it operates as a monomeric protein to execute its functions. By dephosphorylating toxins it contributes to the defense mechanisms of the intestinal mucosa aiding in the preservation of a balanced intestinal environment.

Pathways

Intestinal alkaline phosphatase is integrated into the digestive and immune pathways. Its function is closely linked with the regulation of the Toll-like receptor signaling pathway by detoxifying LPS a major outer membrane component of Gram-negative bacteria. Through this pathway it associates with proteins such as TLR4 thereby influencing immune responses and cytokine production. Additionally it plays a role in the absorption of nutrients by interacting with other digestive enzymes contributing to efficient nutrient uptake and utilization.

Intestinal alkaline phosphatase has been implicated in conditions such as inflammatory bowel disease (IBD) and metabolic syndrome. In inflammatory bowel disease the enzyme's ability to dephosphorylate and detoxify LPS is significant in ameliorating intestinal inflammation. Moreover an association exists between inadequate intestinal alkaline phosphatase activity and metabolic syndrome linking it to proteins such as LPS-binding protein (LBP) involved in inflammation and metabolism. Consequently this enzyme emerges as a potential therapeutic target in managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPI

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alkaline phosphatase (intestinal) antibody [HL1268]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

Western blot - Anti-Alkaline phosphatase (intestinal) antibody [HL1268] (AB307798)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise