Rabbit Recombinant Monoclonal LAMP2 antibody - conjugated to Alkaline Phosphatase.
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.016% Magnesium chloride, 0.001% Zinc chloride
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
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The protein expressed by gene LAMP2 plays an important role in chaperone-mediated autophagy, facilitating the lysosomal degradation of proteins in response to various stresses and as part of normal protein turnover, especially for those with a long biological half-life. It functions by binding target proteins like GAPDH and MLLT11, directing them for lysosomal degradation, and is necessary for the fusion of autophagosomes with lysosomes during autophagy. Although cells lacking LAMP2 express normal VAMP8 levels, they fail to accumulate STX17 on autophagosomes, likely explaining the fusion failure between autophagosomes and lysosomes. LAMP2 is essential for the normal degradation of autophagosomal contents and efficient MHCII-mediated presentation of exogenous antigens, as it facilitates lysosomal protein degradation. It is not required for the presentation of endogenous antigens via MHCII. Specifically, isoform LAMP-2C modulates chaperone-mediated autophagy, reduces presentation of endogenous antigens by MHCII, and does not influence the presentation of exogenous and membrane-derived antigens by MHCII. This supplementary information is collated from multiple sources and compiled automatically.
CD107b, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, LAMP2
Rabbit Recombinant Monoclonal LAMP2 antibody - conjugated to Alkaline Phosphatase.
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.016% Magnesium chloride, 0.001% Zinc chloride
This antibody does not react with mouse and rat species in Immunocytochemistry/ Immunofluorescence application.LAMP2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle (PMID: 10212251PubMed:7488019, PubMed:26856698). For better using it in tissue with low expression level, we suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate).
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
LAMP2A also known as lysosome-associated membrane protein 2A functions as a lysosomal marker and plays a role in the lysosome size and structure regulation. Its molecular weight is approximately 45 kDa. This protein is expressed mainly in tissues with high metabolic activity such as liver kidney and heart. LAMP2A is an integral component of the lysosomal membrane and is important for the proper function and maintenance of lysosomes which are cellular organelles involved in the degradation and recycling of macromolecules.
LAMP2A facilitates chaperone-mediated autophagy (CMA) a specific mechanism for the selective degradation of proteins. It is not part of a larger complex but rather acts independently within the lysosomal membrane. This transport functions through recognizing cytosolic proteins containing a KFERQ-like motif which are then translocated into the lysosome for degradation. LAMP2A upholds cellular homeostasis and is important in adapting to cellular stress by facilitating the turnover of damaged or unnecessary proteins.
LAMP2A is intimately involved in the CMA pathway. This pathway is important for regulating protein quality control often linked with other autophagic processes. LAMP2A also interacts with the heat shock cognate protein (HSC70) which assists in unfolding and transporting substrate proteins to lysosomes. Through these interactions LAMP2A helps maintain cellular proteostasis which is essential for cellular function and survival especially during stress conditions.
LAMP2A is connected to lysosomal storage disorders such as Danon disease. This disease is characterized by the accumulation of autophagic vacuoles due to defects in lysosomal function. LAMP2A mutations alter lysosomal degradation capabilities leading to severe cardiomyopathy and myopathy. Additionally LAMP2A has implications in neurodegenerative diseases like Parkinson's disease due to its role in protein degradation pathways. In these contexts malfunction or altered expression of LAMP2A can disrupt cellular clearance processes contributing to pathogenesis along with proteins such as alpha-synuclein.
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