AB133157

Alkaline Phosphatase Anti-S tag antibody

Name: Anti-S tag Alkaline Phosphatase antibody (ab133157) IgG | Abcam
Brand: Abcam Limited
SKU: AB133157

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(1 Publication)

Rabbit Polyclonal S tag antibody - conjugated to Alkaline Phosphatase. Suitable for ELISA, WB and reacts with Tag samples. Cited in 1 publication.

View Alternative Names

KETAAAKFERQHMDS tag antibody, S peptide, S peptide epitope tag

1 Images

Supplier Data

Western blot - Alkaline Phosphatase Anti-S tag antibody (AB133157)

Imaged using stabilized Alkaline Phosphatase substrate.

All lanes:

Western blot - Alkaline Phosphatase Anti-S tag antibody (ab133157) at 1/10000 dilution

Lane 1:

60 kDa S-tagged protein at 0.3 µg

Lane 2:

60 kDa S-tagged protein at 0.01 µg

Lane 3:

60 kDa S-tagged protein at 0.03 µg

Lane 4:

60 kDa S-tagged protein at 0.001 µg

Lane 5:

60 kDa S-tagged protein at 0.003 µg

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Alkaline Phosphatase

Excitation/Emission

Carrier free

Applications

WB, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Starting dilution: 1/2000</p>" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 1mL

Storage buffer

pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 0.88% Sodium chloride, 0.3% Tris, 0.047% Magnesium chloride, 0.0016% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S tag is a peptide sequence derived from ribonuclease A commonly used in molecular biology for protein purification and detection. This tag has a molecular mass of approximately 2 kDa. Researchers often utilize S tags for their small size which minimizes interference with protein function. The S tag is typically expressed as a fusion with the protein of interest which enables it to be used as a versatile tool in a variety of expression systems.

Biological function summary

S tags do not independently participate in cellular functions but instead facilitate experimental procedures. When fused to a protein the S tag allows for simple detection and purification using anti-S tag antibodies. This functionality makes it possible to isolate and study the protein of interest without affecting its native activity significantly. While S tags do not form part of any specific complex they interact with complexes like alkaline phosphatase in experimental applications to enhance detection signals.

Pathways

S tags themselves do not have inherent roles in cellular pathways. However they are used as a tool in studies involving signal transduction or metabolic pathways where their presence aids in tracking or manipulating pathway components. Due to their utility S tags can be connected indirectly to proteins such as ALP (alkaline phosphatase) by enabling pathway mapping in experiments.

S tags do not directly relate to specific conditions but are instrumental in research. For instance they allow scientists to study proteins connected to cancer or metabolic disorders more effectively. In research related to metabolic disorders S-tagged proteins can be linked to alkaline phosphatase helping in investigations to understand the role of alkaline phosphatase in calcium metabolism issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information S tag

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:34704-17 PubMed26430963

2015

The transcription factor c-Fos coordinates with histone lysine-specific demethylase 2A to activate the expression of cyclooxygenase-2.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoli Lu,Yang Yang,Yipeng Du,Lin-Lin Cao,Meiting Li,Changchun Shen,Tianyun Hou,Ying Zhao,Haiying Wang,Dajun Deng,Lina Wang,Qihua He,Wei-Guo Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alkaline Phosphatase Anti-S tag antibody

Western blot - Alkaline Phosphatase Anti-S tag antibody (AB133157)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Properties and storage information

Form

Reconstitution

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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