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Rabbit Polyclonal S tag antibody - conjugated to Alkaline Phosphatase. Suitable for ELISA, WB and reacts with Tag samples.

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Images

Western blot - Alkaline Phosphatase Anti-S tag antibody (AB133157), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alkaline Phosphatase
Storage buffer

pH: 7.4
Preservative: 0.013% Sodium azide
Constituents: 0.88% Sodium chloride, 0.3% Tris, 0.047% Magnesium chloride, 0.0016% Zinc chloride

Form
Lyophilized
Clonality
Polyclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
Tag
Expected
Tested

Expected
Expected

Species
Tag
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Tag
Dilution info
-
Notes

Starting dilution: 1/2000

Associated Products

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Alternative names

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Rabbit Polyclonal S tag antibody - conjugated to Alkaline Phosphatase. Suitable for ELISA, WB and reacts with Tag samples.

Key facts

Isotype
IgG
Conjugation
Alkaline Phosphatase
Form
Lyophilized
Clonality
Polyclonal
Reconstitution
reconstitute with water at 1mL
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The S tag is a peptide sequence derived from ribonuclease A commonly used in molecular biology for protein purification and detection. This tag has a molecular mass of approximately 2 kDa. Researchers often utilize S tags for their small size which minimizes interference with protein function. The S tag is typically expressed as a fusion with the protein of interest which enables it to be used as a versatile tool in a variety of expression systems.

Biological function summary

S tags do not independently participate in cellular functions but instead facilitate experimental procedures. When fused to a protein the S tag allows for simple detection and purification using anti-S tag antibodies. This functionality makes it possible to isolate and study the protein of interest without affecting its native activity significantly. While S tags do not form part of any specific complex they interact with complexes like alkaline phosphatase in experimental applications to enhance detection signals.

Pathways

S tags themselves do not have inherent roles in cellular pathways. However they are used as a tool in studies involving signal transduction or metabolic pathways where their presence aids in tracking or manipulating pathway components. Due to their utility S tags can be connected indirectly to proteins such as ALP (alkaline phosphatase) by enabling pathway mapping in experiments.

Associated diseases and disorders

S tags do not directly relate to specific conditions but are instrumental in research. For instance they allow scientists to study proteins connected to cancer or metabolic disorders more effectively. In research related to metabolic disorders S-tagged proteins can be linked to alkaline phosphatase helping in investigations to understand the role of alkaline phosphatase in calcium metabolism issues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Western blot - Alkaline Phosphatase Anti-S tag antibody (ab133157), expandable thumbnail

    Western blot - Alkaline Phosphatase Anti-S tag antibody (ab133157)

    Imaged using stabilized Alkaline Phosphatase substrate.

    All lanes: Western blot - Alkaline Phosphatase Anti-S tag antibody (ab133157) at 1/10000 dilution

    Lane 1: 60 kDa S-tagged protein at 0.3 µg

    Lane 2: 60 kDa S-tagged protein at 0.01 µg

    Lane 3: 60 kDa S-tagged protein at 0.03 µg

    Lane 4: 60 kDa S-tagged protein at 0.001 µg

    Lane 5: 60 kDa S-tagged protein at 0.003 µg

    Predicted band size: 18 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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