AB126820

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4]

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(14 Publications)

Mouse Monoclonal PPBT antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Alkaline phosphatase, tissue-nonspecific isozyme.

View Alternative Names

AP-TNAP, TNS-ALP, TNSALP, Alkaline phosphatase liver/bone/kidney isozyme, Phosphoamidase, Phosphocreatine phosphatase, ALPL

6 Images

Flow Cytometry - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Flow Cyt

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Flow Cytometry - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Flow cytometric analysis of MCF-7 cells using ab126820 at 1/200 (green) and negative control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

ab126820, at 1/200, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human ovarian cancer tissues by Immunohistochemistry with DAB staining.

ELISA - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

ELISA

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ELISA - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Example of standard curves obtained using ab126820. Control antigen 100ng (black), antigen at 10ng (purple), antigen at 50ng (blue), antigen at 100ng (red)

Lab

Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Lanes 1 - 3 : Merged signal (red and green). Green - ab126820 observed at 80 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab126820 was shown to specifically react with ALPL in wild-type HAP1 cells as signal was lost in ALPL knockout cells. Wild-type and ALPL knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab126820 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820) at 1/500 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

ALPL knockout HAP1 whole cell lysate at 20 µg

Lane 3:

Saos2 whole cell lysate at 20 µg

Predicted band size: 57 kDa

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Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

All lanes:

Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820) at 1/500 dilution

All lanes:

Recombinant Human protein ALPL (AA: 18-502)

Predicted band size: 57 kDa

false

Unknown

Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

All lanes:

Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

NTERA-2 cell lysate

Predicted band size: 57 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2F4

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ELISA, Flow Cyt, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Alkaline phosphatase, tissue-nonspecific isozyme. The exact immunogen used to generate this antibody is proprietary information.

P05186

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR307490-5 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alkaline Phosphatase Tissue Non-Specific also referred to as ALPL or alkaline phosphatase protein functions mechanically as a hydrolase enzyme. It catalyzes the dephosphorylation of molecules contributing to phosphate and mineral metabolism. This enzyme with a molecular mass of approximately 57 kDa exhibits expression in various tissues including liver bone and kidneys. Researchers can use techniques like alkaline phosphatase ELISA and immunohistochemistry to study its expression and localization.

Biological function summary

Alkaline phosphatase in tissues plays a pivotal role in bone mineralization and development. It functions to hydrolyze phosphate groups releasing inorganic phosphate necessary for bone and teeth formation. It forms part of a larger enzyme complex that interacts with extracellular substrates ensuring the continuous supply of phosphate ions. This enzyme in particular impacts cellular processes involving calcification and cellular differentiation.

Pathways

Alkaline phosphatase operates within phosphate metabolism and signaling pathways. Within the skeletal system it forms part of the regulatory mechanism for osteoblast activity and bone formation. It relates to proteins like osteopontin and bone sialoprotein which regulate the mineralization process. These pathways ensure the balance between phosphate ions and mineral deposition in bone tissue.

Alkaline phosphatase abnormalities correlate with hypophosphatasia and rickets. Hypophosphatasia results from mutations in the ALPL gene causing defective bone mineralization. In cases of rickets improper phosphate metabolism can occur due to dysfunctional enzyme activity. The imbalance in alkaline phosphatase activity also connects to disorders like hyperparathyroidism where calcium and phosphate homeostasis is disrupted involving proteins such as parathyroid hormone.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis (PubMed : 12162492, PubMed : 23688511, PubMed : 25982064). Has broad substrate specificity and can hydrolyze a considerable variety of compounds : however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates (PubMed : 12162492, PubMed : 2220817). Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate : it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration (PubMed : 23688511, PubMed : 25982064). Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization : while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity). Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state; it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner (By similarity). Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters (PubMed : 20049532, PubMed : 2220817). Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors (By similarity). Also capable of dephosphorylating microbial products, such as lipopolysaccharides (LPS) as well as other phosphorylated small-molecules, such as poly-inosine : cytosine (poly I : C) (PubMed : 28448526). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle : localizes to the mitochondria of thermogenic fat cells and acts by mediating hydrolysis of N-phosphocreatine to initiate a futile cycle of creatine dephosphorylation and phosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).

See full target information Alkaline phosphatase, tissue-nonspecific isozyme

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Polymers 15: PubMed37376295

2023

Collagen Scaffolds Laden with Human Periodontal Ligament Fibroblasts Promote Periodontal Regeneration in SD Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Tao Chang,Chuan-Ching Lai,Dan-Jae Lin

Communications biology 5:1270 PubMed36402892

2022

Keratin-mediated hair growth and its underlying biological mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Seong Yeong An,Hyo-Sung Kim,So Yeon Kim,Se Young Van,Han Jun Kim,Jae-Hyung Lee,Song Wook Han,Il Keun Kwon,Chul-Kyu Lee,Sun Hee Do,Yu-Shik Hwang

Advanced healthcare materials 12:e2202030 PubMed36300892

2022

An In Vitro Engineered Osteochondral Model as Tool to Study Osteoarthritis Environment.

Applications

Unspecified application

Species

Unspecified reactive species

Annachiara Scalzone,Giorgia Cerqueni,Xiao-Nong Wang,Ana Ferreira-Duarte,Kenny Dalgarno,Monica Mattioli-Belmonte,Piergiorgio Gentile

Cells 10: PubMed34944031

2021

Lamin A/C-Dependent Translocation of Megakaryoblastic Leukemia-1 and β-Catenin in Cyclic Strain-Induced Osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Asmat Ullah Khan,Rongmei Qu,Yuchao Yang,Tingyu Fan,Yan Peng,Bing Sun,Xianshuai Qiu,Shutong Wu,Zetong Wang,Zhitao Zhou,Muhammad Akram Khan,Jingxing Dai,Jun Ouyang

Stem cell research & therapy 12:523 PubMed34620239

2021

Regulation of the integrin αVβ3- actin filaments axis in early osteogenesis of human fibroblasts under cyclic tensile stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Peng,Rongmei Qu,Yanting Feng,Xiaolan Huang,Yuchao Yang,Tingyu Fan,Bing Sun,Asmat Ullah Khan,Shutong Wu,Jingxing Dai,Jun Ouyang

Biomaterials 277:121082 PubMed34464823

2021

Graphene oxide-functionalized nanocomposites promote osteogenesis of human mesenchymal stem cells via enhancement of BMP-SMAD1/5 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong Li,Shiqi Xiang,Zixuan Lin,Eileen N Li,Haruyo Yagi,Guorui Cao,Lauren Yocum,La Li,Tingjun Hao,Katherine K Bruce,Madalyn R Fritch,Huanlong Hu,Bing Wang,Peter G Alexander,Khiam Aik Khor,Rocky S Tuan,Hang Lin

Oncology letters 22:711 PubMed34457066

2021

RNA mA methyltransferase METTL3 promotes colorectal cancer cell proliferation and invasion by regulating Snail expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfan Wen,Guowei Zhang,Yuwen Meng,Lei Zhang,Min Jiang,Zhitao Yu

Frontiers in chemistry 9:699802 PubMed34409016

2021

Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell-Derived Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiping Lu,Yi Zhang,Shan Xiong,Yinghong Zhou,Lan Xiao,Yaping Ma,Yin Xiao,Xin Wang

Biomolecules 11: PubMed34069539

2021

Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolan Huang,Rongmei Qu,Yan Peng,Yuchao Yang,Tingyu Fan,Bing Sun,Asmat Ullah Khan,Shutong Wu,Kuanhai Wei,Chujiang Xu,Jingxing Dai,Jun Ouyang,Shizhen Zhong

Journal of materials chemistry. B 8:9481-9491 PubMed32996551

2020

A novel 3D indirect co-culture system based on a collagen hydrogel scaffold for enhancing the osteogenesis of stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyerim Kim,Shi Huan Han,Yun-Min Kook,Kyung-Mee Lee,Yuan-Zhe Jin,Won-Gun Koh,Jae Hyup Lee,Kangwon Lee

View all publications

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Product promise

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Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4]

Flow Cytometry - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

ELISA - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (AB126820)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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