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BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Polyclonal PPBT antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Alpl aa 50-100 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Predicted | Predicted |
Mouse | Tested | Tested |
Rat | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
This isozyme plays a key role in skeletal mineralization by regulating levels of diphosphate (PPi).
AP-TNAP, TNSALP, Alkaline phosphatase 2, Alkaline phosphatase liver/bone/kidney isozyme, Akp2, Akp-2, Alpl
Rabbit Polyclonal PPBT antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Alpl aa 50-100 conjugated to Keyhole Limpet Haemocyanin.
AP-TNAP, TNSALP, Alkaline phosphatase 2, Alkaline phosphatase liver/bone/kidney isozyme, Akp2, Akp-2, Alpl
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Liquid
Polyclonal
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Alkaline phosphatase in tissues plays a pivotal role in bone mineralization and development. It functions to hydrolyze phosphate groups releasing inorganic phosphate necessary for bone and teeth formation. It forms part of a larger enzyme complex that interacts with extracellular substrates ensuring the continuous supply of phosphate ions. This enzyme in particular impacts cellular processes involving calcification and cellular differentiation.
Alkaline Phosphatase Tissue Non-Specific also referred to as ALPL or alkaline phosphatase protein functions mechanically as a hydrolase enzyme. It catalyzes the dephosphorylation of molecules contributing to phosphate and mineral metabolism. This enzyme with a molecular mass of approximately 57 kDa exhibits expression in various tissues including liver bone and kidneys. Researchers can use techniques like alkaline phosphatase ELISA and immunohistochemistry to study its expression and localization.
Alkaline phosphatase operates within phosphate metabolism and signaling pathways. Within the skeletal system it forms part of the regulatory mechanism for osteoblast activity and bone formation. It relates to proteins like osteopontin and bone sialoprotein which regulate the mineralization process. These pathways ensure the balance between phosphate ions and mineral deposition in bone tissue.
Alkaline phosphatase abnormalities correlate with hypophosphatasia and rickets. Hypophosphatasia results from mutations in the ALPL gene causing defective bone mineralization. In cases of rickets improper phosphate metabolism can occur due to dysfunctional enzyme activity. The imbalance in alkaline phosphatase activity also connects to disorders like hyperparathyroidism where calcium and phosphate homeostasis is disrupted involving proteins such as parathyroid hormone.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of formalin-fixed, paraffin embedded mouse kidney tissue labeling Alkaline Phosphatase with ab203106 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin embedded rat liver tissue labeling Alkaline Phosphatase with ab203106 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
All lanes: Western blot - Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (AB203106) at 1/200 dilution
Lane 1: mouse brain lysate
Lane 2: mouse heart lysate
All lanes: Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 57 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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