Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Rabbit Recombinant Monoclonal ALKBH1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | ICC/IF | IP | |
---|---|---|---|
Human | Tested | Not recommended | Tested |
Mouse | Tested | Not recommended | Expected |
Rat | Tested | Not recommended | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes Milk recommended as blocking agent. |
Species Mouse | Dilution info 1/1000 | Notes Milk recommended as blocking agent. |
Species Rat | Dilution info 1/1000 | Notes Milk recommended as blocking agent. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Dioxygenase that acts as on nucleic acids, such as DNA and tRNA (PubMed:18603530, PubMed:27745969, PubMed:27497299). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:18603530, PubMed:27497299). A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment (PubMed:27745969, PubMed:27497299). Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs (PubMed:27745969). Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation (PubMed:27745969). In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position (PubMed:27497299). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed:27497299). Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA (PubMed:30392959, PubMed:30017583). N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing (By similarity). Demethylates mRNAs containing N(3)-methylcytidine modification (PubMed:31188562). Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity (PubMed:18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites (PubMed:19959401). DNA lyase activity does not require alpha-ketboglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product (PubMed:19959401, PubMed:23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation (By similarity).
Nucleic acid dioxygenase ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, mRNA N(3)-methylcytidine demethylase, ABH, ALKBH1, ALKBH, ABH1
Rabbit Recombinant Monoclonal ALKBH1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Nucleic acid dioxygenase ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, mRNA N(3)-methylcytidine demethylase, ABH, ALKBH1, ALKBH, ABH1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR19215
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Lanes 1 - 3: Merged signal (red and green). Green - ab195376 observed at 43 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab195376 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ALKBH1 knockout cells. Wild-type and ALKBH1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab195376 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: ALKBH1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: A549 whole cell lysate at 20 µg
Predicted band size: 43 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg
Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg
Lane 4: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa
Exposure time: 30s
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376) at 1/1000 dilution
Lane 1: Human fetal kidney lysate at 10 µg
Lane 2: Human colon lysate at 10 µg
Lane 3: Human fetal liver lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa
Exposure time: 3min
ALKBH1 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab195376 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab195376 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: K562 whole cell lysate 10μg (Input).
Lane 2: ab195376 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195376 in K562 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (AB195376)
Predicted band size: 43 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 30 seconds Lane 5-7: 3 minutes.
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376) at 1/1000 dilution
Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 2: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 5: Mouse brain lysate at 10 µg
Lane 6: Mouse spleen lysate at 10 µg
Lane 7: Rat spleen lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376) at 1/1000 dilution
Lane 1: A549 (Human lung carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: A549 (Human lung carcinoma epithelial cell) transfected with siRNA specifically targeting ALKBH1 whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Exposure time: 120s
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com