Rabbit Recombinant Monoclonal ALKBH1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Images
![Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img57269.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376)")
![Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img162815.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376)")
![Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img35037.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376)")
![Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (AB195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--immunoprecipitation-img21571.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (AB195376)")
![Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img31907.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALKBH1 antibody [EPR19215] (AB195376)")
Publications
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- Nature 617:386-3942023A druggable copper-signalling pathway that drives inflammation.Applications:WBReactive species:MouseStéphanie Solier,Sebastian Müller,Tatiana Cañeque,Antoine Versini,Arnaud Mansart,Fabien Sindikubwabo,Leeroy Baron,Laila Emam,Pierre Gestraud,G Dan Pantoș,Vincent Gandon,Christine Gaillet,Ting-Di Wu,Florent Dingli,Damarys Loew,Sylvain Baulande,Sylvère Durand,Valentin Sencio,Cyril Robil,François Trottein,David Péricat,Emmanuelle Näser,Céline Cougoule,Etienne Meunier,Anne-Laure Bègue,Hélène Salmon,Nicolas Manel,Alain Puisieux,Sarah Watson,Mark A Dawson,Nicolas Servant,Guido Kroemer,Djillali Annane,Raphaël RodriguezPubMed 37100912
- Cellular and molecular gastroenterology and hepatology 14:1213-12332022DNA 6mA Demethylase ALKBH1 Orchestrates Fatty Acid Metabolism and Suppresses Diet-Induced Hepatic Steatosis.Applications:WB, ChIPReactive species:MouseLiping Luo,Ya Liu,Paul Nizigiyimana,Mingsheng Ye,Ye Xiao,Qi Guo,Tian Su,Xianghang Luo,Yan Huang,Haiyan ZhouPubMed 36058506
- Neuroscience 501:103-1302022Codon Usage and mRNA Stability are Translational Determinants of Cellular Response to Canonical Ferroptosis Inducers.Applications:WBReactive species:RatSherif Rashad,Shane R Byrne,Daisuke Saigusa,Jingdong Xiang,Yuan Zhou,Liyin Zhang,Thomas J Begley,Teiji Tominaga,Kuniyasu NiizumaPubMed 35987429
- Cell proliferation 55:e131782022Alkbh1-mediated DNA N6-methyladenine modification regulates bone marrow mesenchymal stem cell fate during skeletal aging.Applications:WBReactive species:MouseGuang-Ping Cai,Ya-Lin Liu,Li-Ping Luo,Ye Xiao,Tie-Jian Jiang,Jian Yuan,Min WangPubMed 35018683
- RNA biology 17:1092-11032020The stress specific impact of ALKBH1 on tRNA cleavage and tiRNA generation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSherif Rashad et. al.PubMed 32521209
- Cell death & disease 10:9092019Association of N-methyladenine DNA with plaque progression in atherosclerosis via myocardial infarction-associated transcripts.
- Science (New York, N.Y.) 365:2019A glycine-specific N-degron pathway mediates the quality control of protein -myristoylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard T Timms,Zhiqian Zhang,David Y Rhee,J Wade Harper,Itay Koren,Stephen J ElledgePubMed 31273098
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IP | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Tested | Not recommended | Expected |
| Rat | Tested | Not recommended | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Milk recommended as blocking agent. |
Species Mouse | Dilution info 1/1000 | Notes Milk recommended as blocking agent. |
Species Rat | Dilution info 1/1000 | Notes Milk recommended as blocking agent. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Dioxygenase that acts as on nucleic acids, such as DNA and tRNA (PubMed:18603530, PubMed:27497299, PubMed:27745969). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:18603530, PubMed:27497299). A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment (PubMed:27497299, PubMed:27745969). Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs (PubMed:27745969). Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation (PubMed:27745969). In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position (PubMed:27497299). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed:27497299). Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA (PubMed:30017583, PubMed:30392959). N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing (By similarity). Demethylates mRNAs containing N(3)-methylcytidine modification (PubMed:31188562). Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity (PubMed:18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites (PubMed:19959401). DNA lyase activity does not require alpha-ketboglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product (PubMed:19959401, PubMed:23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation (By similarity).
Alternative names
ABH, ABH1, ALKBH, ALKBH1, Nucleic acid dioxygenase ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, mRNA N(3)-methylcytidine demethylase
Recommended products
Rabbit Recombinant Monoclonal ALKBH1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19215
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img57269.jpg "Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)")
Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
Lanes 1 - 3: Merged signal (red and green). Green - ab195376 observed at 43 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.ab195376 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ALKBH1 knockout cells. Wild-type and ALKBH1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab195376 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: ALKBH1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: A549 whole cell lysate at 20 µg
Predicted band size: 43 kDa
![Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img162815.jpg "Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)")
Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg
Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg
Lane 4: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa
Exposure time: 30s
![Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img35037.jpg "Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)")
Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution
Lane 1: Human fetal kidney lysate at 10 µg
Lane 2: Human colon lysate at 10 µg
Lane 3: Human fetal liver lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa
Exposure time: 3min
![Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (ab195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--immunoprecipitation-img21571.jpg "Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (ab195376)")
Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (ab195376)
ALKBH1 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab195376 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab195376 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: K562 whole cell lysate 10μg (Input).
Lane 2: ab195376 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab195376 in K562 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-ALKBH1 antibody [EPR19215] (ab195376)
Predicted band size: 43 kDa
![Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img31907.jpg "Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)")
Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 30 seconds Lane 5-7: 3 minutes.
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution
Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 2: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 5: Mouse brain lysate at 10 µg
Lane 6: Mouse spleen lysate at 10 µg
Lane 7: Rat spleen lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 44 kDa
![Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img423306.jpg "Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)")
Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376) at 1/1000 dilution
Lane 1: A549 (Human lung carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: A549 (Human lung carcinoma epithelial cell) transfected with siRNA specifically targeting ALKBH1 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 120s
![Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376), expandable thumbnail](https://content.abcam.com/products/images/alkbh1-antibody-epr19215-ab195376--western-blot-img423306.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALKBH1 antibody [EPR19215] (ab195376)")
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