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AB227496

Anti-ALKBH3 antibody

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(4 Publications)

Rabbit Polyclonal ALKBH3 antibody. Suitable for WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALKBH3.

View Alternative Names

ABH3, DEPC1, ALKBH3, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, hABH3

1 Images
Western blot - Anti-ALKBH3 antibody (AB227496)
  • WB

Supplier Data

Western blot - Anti-ALKBH3 antibody (AB227496)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-ALKBH3 antibody (ab227496) at 1/1000 dilution

All lanes:

Rat stomach tissue extract at 50 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ALKBH3. The exact immunogen used to generate this antibody is proprietary information.

Q96Q83

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 26863196, PubMed : 26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 25944111). Has a strong preference for single-stranded DNA (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 20714506). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed : 22055184). Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA (PubMed : 25797601). Also acts on RNA (PubMed : 12594517, PubMed : 16174769, PubMed : 16858410, PubMed : 26863196, PubMed : 26863410). Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed : 26863196, PubMed : 26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed : 16858410, PubMed : 22055184).
See full target information ALKBH3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 14:e1811 PubMed39175405

2024

PUS7-dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxia Chang,Hao Jin,Yun Cui,Feng Yang,Kanghua Chen,Wenjun Kuang,Chunxiao Huo,Zhangqi Xu,Ya Li,Aifu Lin,Bo Yang,Wei Liu,Shanshan Xie,Tianhua Zhou

Journal of cellular and molecular medicine 26:5292-5302 PubMed36098205

2022

LncRNA ALKBH3-AS1 enhances ALKBH3 mRNA stability to promote hepatocellular carcinoma cell proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Qiliang Lu,Hao Wang,Xiangxiang Lei,Qiancheng Ma,Jie Zhao,Wen Sun,Cheng Guo,Dongsheng Huang,Qiuran Xu

Cell discovery 8:25 PubMed35277482

2022

ALKBH3-dependent mA demethylation of Aurora A mRNA inhibits ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Kuang,Hao Jin,Feng Yang,Xiying Chen,Jianzhao Liu,Ting Li,Yongxia Chang,Min Liu,Zhangqi Xu,Chunxiao Huo,Xiaoyi Yan,Yuehong Yang,Wei Liu,Qiang Shu,Shanshan Xie,Tianhua Zhou

Experimental and therapeutic medicine 16:103-112 PubMed29896230

2018

S-adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient receptor protein 3/protein kinase Cβ/C-kinase-activated protein phosphatase-1 inhibitor of 17 kDa signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ge,Tao Han,Xiaoqiu Li,Lili Shan,Jinhuan Zhang,Yan Hong,Yanqiu Xia,Jun Wang,Mingxiao Hou
View all publications
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Product promise

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