Rabbit Polyclonal ALKBH3 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALKBH3.
Images
Publications
Filter by
- Journal of cellular and molecular medicine 26:5292-53022022LncRNA ALKBH3-AS1 enhances ALKBH3 mRNA stability to promote hepatocellular carcinoma cell proliferation and invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiliang Lu,Hao Wang,Xiangxiang Lei,Qiancheng Ma,Jie Zhao,Wen Sun,Cheng Guo,Dongsheng Huang,Qiuran XuPubMed 36098205
- Cell discovery 8:252022ALKBH3-dependent mA demethylation of Aurora A mRNA inhibits ciliogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjun Kuang,Hao Jin,Feng Yang,Xiying Chen,Jianzhao Liu,Ting Li,Yongxia Chang,Min Liu,Zhangqi Xu,Chunxiao Huo,Xiaoyi Yan,Yuehong Yang,Wei Liu,Qiang Shu,Shanshan Xie,Tianhua ZhouPubMed 35277482
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ALKBH3. The exact immunogen used to generate this antibody is proprietary information. Database link Q96Q83
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Rat | Tested |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rhesus monkey | Dilution info - | Notes - |
Target data
Function
Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:20714506). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA (PubMed:25797601). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:16858410, PubMed:26863196, PubMed:26863410). Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:16858410, PubMed:22055184).
Alternative names
ABH3, DEPC1, ALKBH3, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, hABH3
Recommended products
Rabbit Polyclonal ALKBH3 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALKBH3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ALKBH3. The exact immunogen used to generate this antibody is proprietary information. Database link Q96Q83
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-ALKBH3 antibody (ab227496)
12% SDS-PAGE gel.
All lanes: Western blot - Anti-ALKBH3 antibody (ab227496) at 1/1000 dilution
All lanes: Rat stomach tissue extract at 50 µg
Predicted band size: 33 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com