Rabbit Recombinant Monoclonal ALKBH3 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples.
Images
![Western blot - Anti-ALKBH3 antibody [EPR25444-229] (AB307156), expandable thumbnail](https://content.abcam.com/products/images/alkbh3-antibody-epr25444-229-ab307156--western-blot-img404956.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALKBH3 antibody [EPR25444-229] (AB307156)")
![Flow Cytometry (Intracellular) - Anti-ALKBH3 antibody [EPR25444-229] (AB307156), expandable thumbnail](https://content.abcam.com/products/images/alkbh3-antibody-epr25444-229-ab307156--flow-cytometry-intracellular-img404955.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ALKBH3 antibody [EPR25444-229] (AB307156)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | IHC-P | WB | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).
Alternative names
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, hABH3, DEPC1, ABH3, ALKBH3
Recommended products
Rabbit Recombinant Monoclonal ALKBH3 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples.
Alternative names
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, hABH3, DEPC1, ABH3, ALKBH3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR25444-229
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ALKBH3 antibody [EPR25444-229] (ab307156), expandable thumbnail](https://content.abcam.com/products/images/alkbh3-antibody-epr25444-229-ab307156--western-blot-img404956.jpg "Western blot - Anti-ALKBH3 antibody [EPR25444-229] (ab307156)")
Western blot - Anti-ALKBH3 antibody [EPR25444-229] (ab307156)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the higher MW band at approximately 40 kDa (in lane 4) is unknown.
Exposure time: 15 seconds.All lanes: Western blot - Anti-ALKBH3 antibody [EPR25444-229] (ab307156) at 1/1000 dilution
Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg
Lane 2: PC-3 transfected with siRNA specifically targeting ALKBH3 whole cell lysate
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 32 kDa
Exposure time: 15s
![Flow Cytometry (Intracellular) - Anti-ALKBH3 antibody [EPR25444-229] (ab307156), expandable thumbnail](https://content.abcam.com/products/images/alkbh3-antibody-epr25444-229-ab307156--flow-cytometry-intracellular-img404955.jpg "Flow Cytometry (Intracellular) - Anti-ALKBH3 antibody [EPR25444-229] (ab307156)")
Flow Cytometry (Intracellular) - Anti-ALKBH3 antibody [EPR25444-229] (ab307156)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling ALKBH3 with ab307156 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Downloads
Product protocols
- Visit the general protocols
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