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AB307157

Anti-ALKBH3 antibody [EPR25444-229] - BSA and Azide free

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Rabbit Recombinant Monoclonal ALKBH3 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB and reacts with Human, Transfected cell lysate - Human samples.

View Alternative Names

ABH3, DEPC1, ALKBH3, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, hABH3

2 Images
Flow Cytometry (Intracellular) - Anti-ALKBH3 antibody [EPR25444-229] - BSA and Azide free (AB307157)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ALKBH3 antibody [EPR25444-229] - BSA and Azide free (AB307157)

This data was developed using ab307156, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling ALKBH3 with ab307156 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-ALKBH3 antibody [EPR25444-229] - BSA and Azide free (AB307157)
  • WB

Supplier Data

Western blot - Anti-ALKBH3 antibody [EPR25444-229] - BSA and Azide free (AB307157)

This data was developed using ab307156, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The identity of the higher MW band at approximately 40 kDa (in lane 4) is unknown. Exposure time : 15 seconds.

All lanes:

Western blot - Anti-ALKBH3 antibody [EPR25444-229] (<a href='/en-us/products/primary-antibodies/alkbh3-antibody-epr25444-229-ab307156'>ab307156</a>) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg

Lane 2:

PC-3 transfected with siRNA specifically targeting ALKBH3 whole cell lysate

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 32 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25444-229

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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  • 4
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 26863196, PubMed : 26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 25944111). Has a strong preference for single-stranded DNA (PubMed : 12486230, PubMed : 12594517, PubMed : 16174769, PubMed : 20714506). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed : 22055184). Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA (PubMed : 25797601). Also acts on RNA (PubMed : 12594517, PubMed : 16174769, PubMed : 16858410, PubMed : 26863196, PubMed : 26863410). Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed : 26863196, PubMed : 26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed : 16858410, PubMed : 22055184).
See full target information ALKBH3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com