AB113500

Anti-ALKBH4 antibody

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(1 Publication)

Rabbit Polyclonal ALKBH4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALKBH4 aa 1-300.

View Alternative Names

ABH4, ALKBH4, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, Alkylated DNA repair protein alkB homolog 4, DNA N6-methyl adenine demethylase ALKBH4, Lysine-specific demethylase ALKBH4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH4 antibody (AB113500)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH4 antibody (AB113500)

ab113500, at 1/100 dilution, staining ALKBH4 in Formalin-fixed, Paraffin-embedded Human fetal pancreas tissue by Immunohistochemistry.
Note cytoplasmic and nuclear staining.

Unknown

Western blot - Anti-ALKBH4 antibody (AB113500)

All lanes:

Western blot - Anti-ALKBH4 antibody (ab113500) at 1/500 dilution

Lane 1:

Human fetal spleen lysate

Lane 2:

Human fetal liver lysate

Lane 3:

Human fetal kidney lysate

Predicted band size: 34 kDa

Observed band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ALKBH4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9NXW9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes (PubMed : 23673617). Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed : 23673617). In addition to proteins, also demethylates DNA : specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity).

See full target information ALKBH4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 32:1412-1419 PubMed37260420

2023

Divergent in situ expression of IL-31 and IL-31RA between bullous pemphigoid and pemphigus vulgaris.

Applications

Unspecified application

Species

Unspecified reactive species

Ecem Zeliha Ergun,Rui Aoki,Orsolya N Horváth,Daniela Hartmann,Takashi K Satoh,Laura Calabrese,Ayşe Esra Koku Aksu,Mehmet Salih Gürel,Vildan Manav,Michael J Flaig,Miklós Sárdy,Thomas Ruzicka,Lars E French,Işın Sinem Bağcı

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALKBH4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH4 antibody (AB113500)

Western blot - Anti-ALKBH4 antibody (AB113500)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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