AB195379

Anti-ALKBH4 antibody [EPR19216]

Name: Anti-ALKBH4 antibody [EPR19216] (ab195379) | Abcam
Brand: Abcam Limited
SKU: AB195379
Price: 380 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal ALKBH4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

ABH4, ALKBH4, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, Alkylated DNA repair protein alkB homolog 4, DNA N6-methyl adenine demethylase ALKBH4, Lysine-specific demethylase ALKBH4

3 Images

Supplier Data

Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (AB195379)

ALKBH4 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab195379 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab195379 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HEK-293 whole cell lysate 10ug (Input).
Lane 2 : ab195379 IP in HEK-293 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab195379 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (ab195379)

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Blocking/Dilution : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 33 kDa

true

Exposure time: 1min

Supplier Data

Western blot - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Blocking/Dilution : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 4:

Human pancreas tissue lysate at 10 µg

Lane 5:

WI-38 (human fetal lung fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 33 kDa

true

Exposure time: 1min

Carrier free

Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19216

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Primates": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes (PubMed : 23673617). Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed : 23673617). In addition to proteins, also demethylates DNA : specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity).

See full target information ALKBH4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113520 PubMed41069850

2025

ALKBH5-IGF2BP2 axis mediates prostate cancer progression and docetaxel resistance via m6A-stabilized CLSPN RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Chen,Yang Li,Zongzhu Liang,Zhao Yang,Haotian Ren,Wanqing Wei,Yuanjie Niu,Zhiqun Shang

Scientific reports 11:8677 PubMed33883577

2021

ALKBH4 promotes tumourigenesis with a poor prognosis in non-small-cell lung cancer.

Applications

Species

Human

Kentaro Jingushi,Masaya Aoki,Kazuhiro Ueda,Takahiro Kogaki,Masaya Tanimoto,Yuya Monoe,Masayuki Ando,Takuya Matsumoto,Kentaro Minami,Yuko Ueda,Kaori Kitae,Hiroaki Hase,Toshiyuki Nagata,Aya Harada-Takeda,Masatatsu Yamamoto,Kohichi Kawahara,Kazuhiro Tabata,Tatsuhiko Furukawa,Masami Sato,Kazutake Tsujikawa

EBioMedicine 59:102955 PubMed32853985

2020

N6-Methyladenosine modification of the TRIM7 positively regulates tumorigenesis and chemoresistance in osteosarcoma through ubiquitination of BRMS1.

Applications

Unspecified application

Species

Unspecified reactive species

Chenliang Zhou,Zhichang Zhang,Xiaoshi Zhu,Guowei Qian,Yan Zhou,Yong Sun,Wenxi Yu,Jiahui Wang,Haiyang Lu,Feng Lin,Zan Shen,Shuier Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALKBH4 antibody [EPR19216]

Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Western blot - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Western blot - Anti-ALKBH4 antibody [EPR19216] (AB195379)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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