Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (AB251206)

This data was developed using ab195379, the same antibody clone in a different buffer formulation.

ALKBH4 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab195379 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab195379 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HEK-293 whole cell lysate 10ug (Input).
Lane 2 : ab195379 IP in HEK-293 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab195379 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] (<a href='/en-us/products/primary-antibodies/alkbh4-antibody-epr19216-ab195379'>ab195379</a>)

Predicted band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (AB251206)

This data was developed using ab195379, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ALKBH4 antibody [EPR19216] (<a href='/en-us/products/primary-antibodies/alkbh4-antibody-epr19216-ab195379'>ab195379</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 33 kDa

true

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (AB251206)

This data was developed using ab195379, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ALKBH4 antibody [EPR19216] (<a href='/en-us/products/primary-antibodies/alkbh4-antibody-epr19216-ab195379'>ab195379</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 4:

Human pancreas tissue lysate at 10 µg

Lane 5:

WI-38 (human fetal lung fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 33 kDa

true

Exposure time: 1min