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AB113512

Anti-ALKBH8 antibody

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(2 Publications)

Rabbit Polyclonal ALKBH8 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALKBH8 aa 1-250.

View Alternative Names

ABH8, ALKBH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8, Alkylated DNA repair protein alkB homolog 8, Alpha-ketoglutarate-dependent dioxygenase ALKBH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ALKBH8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH8 antibody (AB113512)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH8 antibody (AB113512)

ab113512 at 1/100 dilution staining ALKBH8 in Formalin-fixed, Paraffin-embedded Human fetal spleen tissue by Immunohistochemistry.
Note cytoplasmic and nuclear staining.

Western blot - Anti-ALKBH8 antibody (AB113512)
  • WB

Unknown

Western blot - Anti-ALKBH8 antibody (AB113512)

All lanes:

Western blot - Anti-ALKBH8 antibody (ab113512) at 1/500 dilution

Lane 1:

Human fetal spleen lysate

Lane 2:

A549 cell lysate

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ALKBH8 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q96BT7

Specificity

ab113512 is also predicted to react with isoform 4 of Human ALKBH8 (76kDa).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab113512 is purified by a protein affinity column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed : 20123966, PubMed : 20308323, PubMed : 31079898). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed : 20123966, PubMed : 20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys) (PubMed : 20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed : 21285950). Required for normal survival after DNA damage (PubMed : 20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed : 19293182).
See full target information ALKBH8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

RNA (New York, N.Y.) 28:1568-1581 PubMed36192131

2022

HITS-CLIP analysis of human ALKBH8 reveals interactions with fully processed substrate tRNAs and with specific noncoding RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Cavallin,Marek Bartosovic,Tomas Skalicky,Praveenkumar Rengaraj,Martin Demko,Martina Christina Schmidt-Dengler,Aleksej Drino,Mark Helm,Stepanka Vanacova

The Journal of experimental medicine 213:2503-2523 PubMed27811057

2016

Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvain Delaunay,Francesca Rapino,Lars Tharun,Zhaoli Zhou,Lukas Heukamp,Martin Termathe,Kateryna Shostak,Iva Klevernic,Alexandra Florin,Hadrien Desmecht,Christophe J Desmet,Laurent Nguyen,Sebastian A Leidel,Anne E Willis,Reinhard Büttner,Alain Chariot,Pierre Close
View all publications
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Product promise

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