JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB320759

Anti-Allergin 1 antibody [EPR27238A-M2] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Multiclonal Allergin 1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

C17orf60, MCA32, MILR1, Allergin-1, Allergy inhibitory receptor 1, Mast cell antigen 32, Mast cell immunoglobulin-like receptor 1, MCA-32

1 Images
Sandwich ELISA - Anti-Allergin 1 antibody [EPR27238A-M2] - BSA and Azide free (Detector) (AB320759)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Allergin 1 antibody [EPR27238A-M2] - BSA and Azide free (Detector) (AB320759)

Sandwich ELISA of ab320757 with the capture antibody ab320758 dilution at 2 ug/mL and detector antibody ab320759 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

EPR27238A-M2

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab320759 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Allergin 1. The recommended pair for sandwich ELISA is:

Capture: ab320758, Human Allergin 1 Capture Antibody (unconjugated)

Detector: ab320759, Human Allergin 1 Detector Antibody (unconjugated)

The reference range value is 39.06-2500 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Allergin-1 also known as allergine or epityphlon is a membrane protein expressed in myeloid cells particularly mast cells and basophils. The protein has a mass of approximately 50 kDa. Allergin-1 plays a role in signaling processes that regulate immune responses especially those involving allergen recognition and response. This protein contributes to modulating the activation and deactivation of these immune cells helping maintain a balanced immune reaction.
Biological function summary

Allergin-1 functions as an inhibitory receptor on myeloid cells. It forms part of a complex with other signaling proteins to suppress excessive immune responses that can lead to allergic reactions. This modulation involves interacting with several intracellular signaling proteins which in turn influence the release of mediators that affect the immune response. Allergin-1 through its inhibitory actions helps prevent over-activation of immune pathways that can contribute to allergic inflammation.

Pathways

Allergin-1 is involved in immune regulatory pathways such as the Fc receptor signaling pathway. It interacts with proteins like Syk kinase and SH2-containing phosphatases contributing to the negative regulation of cell activation. These pathways are key in the immune system's ability to distinguish between harmful and benign stimuli playing a protective role against unwarranted inflammatory responses.

Allergin-1 has connections to allergic conditions such as asthma and atopic dermatitis. Allergin-1's ability to modulate immune cell activation can influence the severity and occurrence of these allergic diseases. The protein’s interaction with other immune regulators such as SHIP1 demonstrates its importance in managing diseases where exaggerated immune responses occur. Understanding these connections is essential for developing therapeutic strategies aimed at treating allergic disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells. Negatively regulates IgE-mediated mast cell activation and suppresses the type I immediate hypersensitivity reaction (By similarity).
See full target information MILR1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Proteins & Peptides

AB277018

Recombinant Mouse Allergin 1 protein (Fc Chimera)

SDS-PAGE - Recombinant Mouse Allergin 1 protein (Fc Chimera) (AB277018)

  • 0
  • 0
Primary Antibodies

AB151707

Anti-CD204 antibody [EPR7536]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-CD204 antibody [EPR7536] (AB151707)

  • 0
  • 0
Primary Antibodies

AB91606

Anti-Lysophospholipase 1/LPL-I antibody [EPR3667]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (AB91606)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com