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AB121785

Anti-ALOX12B antibody

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(3 Publications)

Rabbit Polyclonal ALOX12B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Arachidonate 12-lipoxygenase, 12R-type aa 400-550.

View Alternative Names

12R-LOX, 12R-lipoxygenase, Epidermis-type lipoxygenase 12, ALOX12B

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ALOX12B antibody (AB121785)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ALOX12B antibody (AB121785)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121785 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALOX12B antibody (AB121785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALOX12B antibody (AB121785)

ab121785, at 1/10 dilution, staining ALOX12B in paraffin-embedded Human skin tissue, by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Arachidonate 12-lipoxygenase, 12R-type aa 400-550. The exact immunogen used to generate this antibody is proprietary information.

O75342

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Complementary Products

Primary Antibodies

AB168384

Anti-12 Lipoxygenase/ALOX12 antibody [EPR11380(B)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-12 Lipoxygenase/ALOX12 antibody [EPR11380(B)] (AB168384)

  • 0
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Proteins & Peptides

AB157877

Recombinant Human ALOX12B protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ALOX12B protein (GST tag N-Terminus) (AB157877)

  • 0
  • 0
Primary Antibodies

AB244205

Anti-15 Lipoxygenase 1 antibody [EPR22138]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15 Lipoxygenase 1 antibody [EPR22138] (AB244205)

  • 0
  • 0
Primary Antibodies

AB118800

Anti-ZZZ3 antibody

Immunoprecipitation - Anti-ZZZ3 antibody (AB118800)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed : 21558561, PubMed : 9618483, PubMed : 9837935). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed : 21558561). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed : 21558561). May also play a role in the regulation of the expression of airway mucins (PubMed : 22441738).
See full target information Arachidonate 12-lipoxygenase, 12R-type
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:157 PubMed38733530

2024

circPDK1 competitively binds miR-4731-5p to mediate GIGYF1 expression and increase paclitaxel sensitivity in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

YunYin Feng,TaoLong Zhang,Hong Liu

Frontiers in cell and developmental biology 9:684259 PubMed34307361

2021

Development and Validation of a Ferroptosis-Related Gene Signature for Overall Survival Prediction in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Tian,Yan Zhou,Lizhe Zhu,Huan Gao,Jin Yang

The British journal of dermatology 173:285-7 PubMed25524567

2015

A severe collodion phenotype in the newborn period associated with a homozygous missense mutation in ALOX12B.

Applications

Unspecified application

Species

Unspecified reactive species

P J Bland,C Chronnell,V Plagnol,H Kayserili,D P Kelsell
View all publications
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Product promise

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