Rabbit Polyclonal ALP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDLIM3 aa 1-100.
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- Journal of nanobiotechnology 21:4852023Reprogramming mitochondrial metabolism of macrophages by miRNA-released microporous coatings to prevent peri-implantitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongming Zhang,Yun Yuan,Hanxiao Xue,Runping Yu,Xiayue Jin,Xiaolin Wu,Hui HuangPubMed 38105216
- Experimental and therapeutic medicine 26:5312023FRZB affects aureus‑induced osteomyelitis in human bone marrow derived stem cells by regulating the Wnt/β‑catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Li,Wenyong Pang,Hongsong Fan,Hao Wang,Leibing ZhangPubMed 37869648
- Iranian journal of basic medical sciences 26:785-7902023Therapeutic effects of diosgenin on alveolar bone loss and apoptosis in diabetic rats with experimental periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlper Kızıldağa,Aysan Lektemür Alpanb,Melih Özdedec,Tuğba Aydınd,Özlem ÖzmenePubMed 37396943
- International journal of nanomedicine 18:2019-20352023Fabrication of Interleukin-4 Encapsulated Bioactive Microdroplets for Regulating Inflammation and Promoting Osteogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhang,Jin Cao,Minghui Jian,Zhixiao Zhou,Nadia Anwar,Lan Xiao,Yaping Ma,Dingmei Zhang,Jun Zhang,Xin WangPubMed 37155503
- Journal of orthopaedic translation 38:175-1892022Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Sun,Huiqiao Wu,Jiajia Lu,Rongcheng Zhang,Xiaolong Shen,Yifei Gu,Changgui Shi,Ying Zhang,Wen YuanPubMed 36439629
- Journal of biological engineering 16:222022A tailored bioactive 3D porous poly(lactic-acid)-exosome scaffold with osteo-immunomodulatory and osteogenic differentiation properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhang,Mengjie Huo,Yi Wang,Lan Xiao,Jianmei Wu,Yaping Ma,Dingmei Zhang,Xuemei Lang,Xin WangPubMed 35996115
- Regenerative therapy 21:175-1842022Circ_0018168 inhibits the proliferation and osteogenic differentiation of fibroblasts in ankylosing spondylitis via regulating miR-330-3p/DKK1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhao,Jiaxun Jiao,Guanghui Yan,Wei Wei,Genqiang Fang,Tiemiao YuPubMed 35891711
- Journal of evolutionary biochemistry and physiology 58:430-4402022Evaluation of the Effects of Favipiravir Combined with Vitamin C on Alveolar Bone in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Lektemur Alpan,G Torumtay Cin,Ö Özmen,M F Doğan,Y ŞahinPubMed 35599639
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e21059092022Human Mesenchymal Stem Cell-Derived Miniature Joint System for Disease Modeling and Drug Testing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhong Li,Zixuan Lin,Silvia Liu,Haruyo Yagi,Xiurui Zhang,Lauren Yocum,Monica Romero-Lopez,Claire Rhee,Meagan J Makarcyzk,Ilhan Yu,Eileen N Li,Madalyn R Fritch,Qi Gao,Kek Boon Goh,Benjamen O'Donnell,Tingjun Hao,Peter G Alexander,Bhushan Mahadik,John P Fisher,Stuart B Goodman,Bruce A Bunnell,Rocky S Tuan,Hang LinPubMed 35436042
- Pharmaceutics 13:20213D GelMA ICC Scaffolds Combined with SW033291 for Bone Regeneration by Modulating Macrophage Polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Jiang,Guo Bai,Xin Liu,Yuxiao Chen,Guangzhou Xu,Chi Yang,Zhiyuan ZhangPubMed 34834349
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PDLIM3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q53GG5
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
May play a role in the organization of actin filament arrays within muscle cells.
Alternative names
ALP, PDLIM3, PDZ and LIM domain protein 3, Actinin-associated LIM protein, Alpha-actinin-2-associated LIM protein
Recommended products
Rabbit Polyclonal ALP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDLIM3 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PDLIM3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q53GG5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody (ab224335)
Paraffin-embedded human skeletal muscle tissue stained for ALP using ab224335 at 1/200 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-ALP antibody (ab224335)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ALP (green) using ab224335 at 4 μg/ml in ICC/IF.
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