Anti-ALP antibody [EPR27191-14] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)

This data was developed using ab307726, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALP with ab307726 at 1/1000 (0.254 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control : no staining on the human kidney (PMID : 9334352). The section was incubated with ab307726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)

This data was developed using ab307726, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ALP with ab307726 at 1/1000 (0.254 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human skeletal muscle (PMID : 9334352). The section was incubated with ab307726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• WB

Lab

Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)

This data was developed using ab307726, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : human kidney (PMID : 9334352). The bands beneath the target band (43 kDa) are likely to be degraded target fragments. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-ALP antibody [EPR27191-14] (<a href='/en-us/products/primary-antibodies/alp-antibody-epr27191-14-ab307726'>ab307726</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 43 kDa

false

• WB

Lab

Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)

This data was developed using ab307726, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The bands beneath the target band (43 kDa) are likely to be degraded target fragments. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 10 seconds

All lanes:

Western blot - Anti-ALP antibody [EPR27191-14] (<a href='/en-us/products/primary-antibodies/alp-antibody-epr27191-14-ab307726'>ab307726</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human PDLIM3 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 43 kDa

false

Exposure time: 10s