Rabbit Recombinant Monoclonal ALP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727), expandable thumbnail](https://content.abcam.com/products/images/alp-antibody-epr27191-14-bsa-and-azide-free-ab307727--western-blot-img407416.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)")
![Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727), expandable thumbnail](https://content.abcam.com/products/images/alp-antibody-epr27191-14-bsa-and-azide-free-ab307727--western-blot-img407415.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727), expandable thumbnail](https://content.abcam.com/products/images/alp-antibody-epr27191-14-bsa-and-azide-free-ab307727--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407190.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727), expandable thumbnail](https://content.abcam.com/products/images/alp-antibody-epr27191-14-bsa-and-azide-free-ab307727--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407189.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
May play a role in the organization of actin filament arrays within muscle cells.
Alternative names
ALP, PDLIM3, PDZ and LIM domain protein 3, Actinin-associated LIM protein, Alpha-actinin-2-associated LIM protein
Recommended products
Rabbit Recombinant Monoclonal ALP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27191-14
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727), expandable thumbnail](https://content.abcam.com/products/images/alp-antibody-epr27191-14-bsa-and-azide-free-ab307727--western-blot-img407416.jpg "Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727)")
Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727)
This data was developed using Anti-ALP antibody [EPR27191-14] ab307726, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands beneath the target band (43 kDa) are likely to be degraded target fragments. In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
Exposure time: 10 secondsAll lanes: Western blot - Anti-ALP antibody [EPR27191-14] (Anti-ALP antibody [EPR27191-14] ab307726) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human PDLIM3 expression vector containing a His-tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 43 kDa
Exposure time: 10s
![Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727), expandable thumbnail](https://content.abcam.com/products/images/alp-antibody-epr27191-14-bsa-and-azide-free-ab307727--western-blot-img407415.jpg "Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727)")
Western blot - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727)
This data was developed using Anti-ALP antibody [EPR27191-14] ab307726, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: human kidney (PMID: 9334352).
The bands beneath the target band (43 kDa) are likely to be degraded target fragments. In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-ALP antibody [EPR27191-14] (Anti-ALP antibody [EPR27191-14] ab307726) at 1/1000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727), expandable thumbnail](https://content.abcam.com/products/images/alp-antibody-epr27191-14-bsa-and-azide-free-ab307727--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407190.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727)
This data was developed using Anti-ALP antibody [EPR27191-14] ab307726, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALP with Anti-ALP antibody [EPR27191-14] ab307726 at 1/1000 (0.254 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Negative control: no staining on the human kidney (PMID: 9334352).
The section was incubated with Anti-ALP antibody [EPR27191-14] ab307726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727), expandable thumbnail](https://content.abcam.com/products/images/alp-antibody-epr27191-14-bsa-and-azide-free-ab307727--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407189.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (ab307727)
This data was developed using Anti-ALP antibody [EPR27191-14] ab307726, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ALP with Anti-ALP antibody [EPR27191-14] ab307726 at 1/1000 (0.254 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Positive staining on human skeletal muscle (PMID: 9334352).
The section was incubated with Anti-ALP antibody [EPR27191-14] ab307726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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