Rabbit Recombinant Monoclonal Alpha-1-acid glycoprotein 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (AB248656), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-acid-glycoprotein-agp-antibody-epr5605-bsa-and-azide-free-ab248656--western-blot-img67177.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (AB248656)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (AB248656), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-acid-glycoprotein-agp-antibody-epr5605-bsa-and-azide-free-ab248656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41090.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (AB248656)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Alternative names
AGP1, ORM1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1
Recommended products
Rabbit Recombinant Monoclonal Alpha-1-acid glycoprotein 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5605
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248656 is the carrier-free version of Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] ab134042.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.
- Biological function summary
AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.
- Pathways
Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.
- Associated diseases and disorders
AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (ab248656), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-acid-glycoprotein-agp-antibody-epr5605-bsa-and-azide-free-ab248656--western-blot-img67177.jpg "Western blot - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (ab248656)")
Western blot - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (ab248656)
This data was developed using Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] ab134042, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] (Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] ab134042) at 1/1000 dilution
Lane 1: Human small intestine lysate at 10 µg
Lane 2: Human plasma at 10 µg
Lane 3: Human testis lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (ab248656), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-acid-glycoprotein-agp-antibody-epr5605-bsa-and-azide-free-ab248656--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41090.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (ab248656)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] - BSA and Azide free (ab248656)
This data was developed using Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] ab134042, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Alpha-1-Acid Glycoprotein / AGP with Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR5605] ab134042 at 1/250 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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