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AB313577

Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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Rabbit Recombinant Monoclonal alpha 1d Adrenergic Receptor antibody. Suitable for IHC-P, WB and reacts with Mouse, Transfected cell line - Mouse samples.

View Alternative Names

Adra1a, Gpcr8, Adra1d, Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells tissue labeling alpha 1 Adrenergic Receptor with ab313577 at 1/10000 (0.051 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on (A) HEK-293T cells transfected with a mouse ADRA1D expression vector containing a his tag, almost no staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling alpha 1 Adrenergic Receptor with ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse cerebrum. The section was incubated with ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling alpha 1 Adrenergic Receptor with ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse cerebellum. The section was incubated with ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling alpha 1 Adrenergic Receptor with ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse kidney. The section was incubated with ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)
  • WB

Supplier Data

Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (AB313577)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver (PMID : 9326654). Low expression : hypothalamus (PMID : 9285356). 120kDa (dimer) is due to polymerization (PMID : 27382054). Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (ab313577) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 40 µg

Lane 2:

Mouse cerebellum tissue lysate at 40 µg

Lane 3:

Mouse hypothalamus tissue lysate at 40 µg

Lane 4:

Mouse liver tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 120 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27512-50

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell line - Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The alpha 1d adrenergic receptor also known as the alpha-1d adrenoceptor is a G-protein-coupled receptor with mass approximately 70 kDa. It plays a critical role in the modulation of physiological responses by binding catecholamines like norepinephrine and epinephrine. Alpha-1d adrenergic receptor is widely expressed in vascular smooth muscle heart liver and central nervous system. This receptor remains an important target for pharmacological research due to its involvement in various physiological processes.
Biological function summary

The alpha-1d adrenergic receptor promotes vasoconstriction and increases blood pressure through the activation of phospholipase C pathway resulting in calcium release from intracellular stores. It functions as part of a receptor complex that interacts with other alpha-1 adrenergic subtypes such as alpha-1a and alpha-1b adrenergic receptors differentiating their roles in tissue-specific actions. These receptor activities affect muscle contraction and influence systemic vascular resistance.

Pathways

This adrenergic receptor is engaged in the adrenergic signaling in cardiomyocytes and vascular smooth muscle contraction pathways. It facilitates communication between the signaling molecules and cellular responses. The alpha-1d adrenergic receptor partners with proteins like Gq/11 protein family which mediates signal transduction leading to its physiological effects. Interaction with other receptors such as beta-adrenergic receptors shapes the overall cardiovascular outcomes by providing a balance between constriction and relaxation.

The alpha-1d adrenergic receptor links to hypertension and benign prostatic hyperplasia where its modulation can influence disease progression and treatment outcomes. In hypertension dysregulation of alpha-1d adrenergic signaling may alter vascular tone and resistance contributing to elevated blood pressure. Connections with proteins such as phospholipase C confirm its involvement in the signaling cascade that affects these conditions. Modulation of this receptor activity offers therapeutic potential for related cardiovascular and urological disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
See full target information Adra1d
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Product promise

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