Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)

This data was developed using ab313577, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells tissue labeling alpha 1 Adrenergic Receptor with ab313577 at 1/10000 (0.051 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on (A) HEK-293T cells transfected with a mouse ADRA1D expression vector containing a his tag, almost no staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)

This data was developed using ab313577, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling alpha 1 Adrenergic Receptor with ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse cerebrum. The section was incubated with ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)

This data was developed using ab313577, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling alpha 1 Adrenergic Receptor with ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse cerebellum. The section was incubated with ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)

This data was developed using ab313577, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling alpha 1 Adrenergic Receptor with ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse kidney. The section was incubated with ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)

This data was developed using ab313577, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver (PMID : 9326654). Low expression : hypothalamus (PMID : 9285356). 120kDa (dimer) is due to polymerization (PMID : 27382054). Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (<a href='/en-us/products/primary-antibodies/alpha-1-adrenergic-receptor-antibody-epr27512-50-ab313577'>ab313577</a>) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 40 µg

Lane 2:

Mouse cerebellum tissue lysate at 40 µg

Lane 3:

Mouse hypothalamus tissue lysate at 40 µg

Lane 4:

Mouse liver tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 120 kDa

false

Exposure time: 180s