Rabbit Recombinant Monoclonal alpha 1d Adrenergic Receptor antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Transfected cell line - Mouse samples.
Images
![Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--western-blot-img418202.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418264.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418263.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418262.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418261.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (AB313578)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | IHC-Fr | Flow Cyt | IP | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Alternative names
Adra1a, Gpcr8, Adra1d, Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor
Recommended products
Rabbit Recombinant Monoclonal alpha 1d Adrenergic Receptor antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Transfected cell line - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27512-50
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The alpha 1d adrenergic receptor also known as the alpha-1d adrenoceptor is a G-protein-coupled receptor with mass approximately 70 kDa. It plays a critical role in the modulation of physiological responses by binding catecholamines like norepinephrine and epinephrine. Alpha-1d adrenergic receptor is widely expressed in vascular smooth muscle heart liver and central nervous system. This receptor remains an important target for pharmacological research due to its involvement in various physiological processes.
- Biological function summary
The alpha-1d adrenergic receptor promotes vasoconstriction and increases blood pressure through the activation of phospholipase C pathway resulting in calcium release from intracellular stores. It functions as part of a receptor complex that interacts with other alpha-1 adrenergic subtypes such as alpha-1a and alpha-1b adrenergic receptors differentiating their roles in tissue-specific actions. These receptor activities affect muscle contraction and influence systemic vascular resistance.
- Pathways
This adrenergic receptor is engaged in the adrenergic signaling in cardiomyocytes and vascular smooth muscle contraction pathways. It facilitates communication between the signaling molecules and cellular responses. The alpha-1d adrenergic receptor partners with proteins like Gq/11 protein family which mediates signal transduction leading to its physiological effects. Interaction with other receptors such as beta-adrenergic receptors shapes the overall cardiovascular outcomes by providing a balance between constriction and relaxation.
- Associated diseases and disorders
The alpha-1d adrenergic receptor links to hypertension and benign prostatic hyperplasia where its modulation can influence disease progression and treatment outcomes. In hypertension dysregulation of alpha-1d adrenergic signaling may alter vascular tone and resistance contributing to elevated blood pressure. Connections with proteins such as phospholipase C confirm its involvement in the signaling cascade that affects these conditions. Modulation of this receptor activity offers therapeutic potential for related cardiovascular and urological disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--western-blot-img418202.jpg "Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)")
Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)
This data was developed using Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver (PMID: 9326654).
Low expression: hypothalamus (PMID: 9285356).
120kDa (dimer) is due to polymerization (PMID: 27382054).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] (Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577) at 1/1000 dilution
Lane 1: Mouse cerebral cortex tissue lysate at 40 µg
Lane 2: Mouse cerebellum tissue lysate at 40 µg
Lane 3: Mouse hypothalamus tissue lysate at 40 µg
Lane 4: Mouse liver tissue lysate at 40 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 120 kDa
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418264.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)
This data was developed using Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells tissue labeling alpha 1 Adrenergic Receptor with Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577 at 1/10000 (0.051 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on (A) HEK-293T cells transfected with a mouse ADRA1D expression vector containing a his tag, almost no staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418263.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)
This data was developed using Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling alpha 1 Adrenergic Receptor with Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse kidney. The section was incubated with Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418262.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)
This data was developed using Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling alpha 1 Adrenergic Receptor with Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse cerebellum. The section was incubated with Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-adrenergic-receptor-antibody-epr27512-50-bsa-and-azide-free-ab313578--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418261.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] - BSA and Azide free (ab313578)
This data was developed using Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling alpha 1 Adrenergic Receptor with Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577 at 1/500 (1.028 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining staining on mouse cerebrum. The section was incubated with Anti-alpha 1 Adrenergic Receptor antibody [EPR27512-50] ab313577 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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