AB230695

Anti-alpha 1 Antitrypsin antibody [11G2]

Name: Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) | Abcam
Brand: Abcam Limited
SKU: AB230695

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(1 Publication)

Mouse Monoclonal alpha 1 Antitrypsin antibody. Suitable for IHC-P, IP, WB and reacts with Human, Dog, African green monkey, Transfected cell lysate - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINA1.

View Alternative Names

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

Formalin-fixed, paraffin-embedded human liver tissue stained for alpha 1 Antitrypsin using ab230695 at 10 μg/ml in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

alpha 1 Antitrypsin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) DDK tagged alpha 1 Antitrypsin antibody overexpression lysate with 2 μg ab230695 and 20 μl (0.1 mg) of goat anti-mouse conjugated magnetic beads. Western blot was performed from the immunoprecipitate using rabbit anti-DDK polyclonal antibody.

Lane 1 : Negative control : IP without adding anti-alpha 1 Antitrypsin antibody in HEK-293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.

Lane 2 : ab230695 IP in -293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.

All lanes:

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY alpha 1 Antitrypsin at 5 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

11G2

Isotype

IgG1

Carrier free

Reacts with

Dog, Human, African green monkey

Applications

IP, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SERPINA1.

P01009

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/µL", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by Protein A/G.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eade9459 PubMed36662852

2023

Inflammation of the retinal pigment epithelium drives early-onset photoreceptor degeneration in -associated retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Maria E Mercau,Yemsratch T Akalu,Francesca Mazzoni,Gavin Gyimesi,Emily J Alberto,Yong Kong,Brian P Hafler,Silvia C Finnemann,Carla V Rothlin,Sourav Ghosh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Antitrypsin antibody [11G2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise