Mouse Monoclonal alpha 1 Antitrypsin antibody. Suitable for IHC-P, IP, WB and reacts with Human, Dog, African green monkey, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINA1.
Images
![Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-11g2-ab230695--western-blot-img100557.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-11g2-ab230695--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117870.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)")
![Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-11g2-ab230695--immunoprecipitation-img61889.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)")
![Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-11g2-ab230695--western-blot-img32605.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (AB230695)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SERPINA1. Database link P01009
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| African green monkey | Expected | Expected | Tested |
| Dog | Expected | Expected | Tested |
| Transfected cell lysate - Human | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Species African green monkey | Dilution info 10 µg/µL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/1000 - 1/2000 | Notes - |
Species Dog | Dilution info 1/1000 - 1/2000 | Notes - |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
Species Transfected cell lysate - Human | Dilution info 1/1000 - 1/2000 | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Alternative names
AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1
Recommended products
Mouse Monoclonal alpha 1 Antitrypsin antibody. Suitable for IHC-P, IP, WB and reacts with Human, Dog, African green monkey, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINA1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SERPINA1. Database link P01009
- Clone number
- 11G2
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.
- Biological function summary
A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.
- Pathways
Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.
- Associated diseases and disorders
Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
![Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-11g2-ab230695--western-blot-img100557.jpg "Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)")
Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)
All lanes: Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg
Lane 2: HEK-293T cell lysate transfected with pCMV6-ENTRY alpha 1 Antitrypsin at 5 µg
Predicted band size: 46 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-11g2-ab230695--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117870.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)
Formalin-fixed, paraffin-embedded human liver tissue stained for alpha 1 Antitrypsin using ab230695 at 10 μg/ml in immunohistochemical analysis.
![Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-11g2-ab230695--immunoprecipitation-img61889.jpg "Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)")
Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)
alpha 1 Antitrypsin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) DDK tagged alpha 1 Antitrypsin antibody overexpression lysate with 2 μg ab230695 and 20 μl (0.1 mg) of goat anti-mouse conjugated magnetic beads. Western blot was performed from the immunoprecipitate using rabbit anti-DDK polyclonal antibody.
Lane 1: Negative control: IP without adding anti-alpha 1 Antitrypsin antibody in HEK-293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.
Lane 2: ab230695 IP in -293T DDK tagged alpha 1 Antitrypsin antibody overexpression lysate.
All lanes: Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)
Predicted band size: 46 kDa
![Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-11g2-ab230695--western-blot-img32605.jpg "Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)")
Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695)
All lanes: Western blot - Anti-alpha 1 Antitrypsin antibody [11G2] (ab230695) at 1/1000 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg
Lane 3: HT-29 (human colorectal adenocarcinoma cell line) cell extract at 35 µg
Lane 4: A549 (human lung carcinoma cell line) cell extract at 35 µg
Lane 5: COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg
Lane 7: MDCK (canine kidney cell line) cell extract at 35 µg
Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg
Predicted band size: 46 kDa
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