AB231093

Anti-alpha 1 Antitrypsin antibody

Name: Anti-alpha 1 Antitrypsin antibody (ab231093) | Abcam
Brand: Abcam Limited
SKU: AB231093

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(2 Publications)

Rabbit Polyclonal A1AT1 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Serpina1a.

View Alternative Names

Dom1, Spi1-1, Serpina1a, Alpha-1-antitrypsin 1-1, AAT, Alpha-1 protease inhibitor 1, Alpha-1-antiproteinase, Serine protease inhibitor 1-1, Serine protease inhibitor A1a, Serpin A1a

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB231093)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB231093)

Paraffin-embedded mouse liver tissue stained for alpha 1 Antitrypsin using ab231093 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB231093)

Formalin-fixed, paraffin-embedded mouse liver tissue stained for alpha 1 Antitrypsin using ab231093 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

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Western blot - Anti-alpha 1 Antitrypsin antibody (ab231093) at 2 µg/mL

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Mouse liver lysate

Predicted band size: 45 kDa,46 kDa,60 kDa,83 kDa,94 kDa

false

Supplier Data

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody (ab231093) at 2 µg/mL

All lanes:

Mouse kidney lysate

Predicted band size: 35 kDa,39 kDa,42 kDa,46 kDa,70 kDa

false

Supplier Data

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody (ab231093) at 2 µg/mL

All lanes:

Mouse testis lysate

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody (ab231093) at 2 µg/mL

All lanes:

Mouse heart lysate

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody (ab231093) at 2 µg/mL

All lanes:

Recombinant mouse alpha 1 Antitrypsin protein

Predicted band size: 100 kDa,46 kDa,47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Serpina1a.

P07758

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231093 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.

See full target information Serpina1a

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:335 PubMed40089779

2025

F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Taile Jing,Cunjin Wu,Minghao Lu,Xiaolin Yao,Dan Xia,Ding Peng

Scientific reports 10:6765 PubMed32317725

2020

The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Bruno Passet,Johan Castille,Samira Makhzami,Sandrine Truchet,Anne Vaiman,Sandrine Floriot,Katayoun Moazami-Goudarzi,Marthe Vilotte,Anne-Laure Gaillard,Louise Helary,Maud Bertaud,Olivier Andréoletti,Daniel Vaiman,Pierre Calvel,Nathalie Daniel-Carlier,Mohammed Moudjou,Christian Beauvallet,Mohamed Benharouga,Denis Laloé,Sophie Mouillet-Richard,Amandine Duchesne,Vincent Béringue,Jean-Luc Vilotte

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Antitrypsin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB231093)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB231093)

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

Western blot - Anti-alpha 1 Antitrypsin antibody (AB231093)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise