AB9373

Anti-alpha 1 Antitrypsin antibody

Name: Anti-alpha 1 Antitrypsin antibody. Rabbit polyclonal (ab9373) | Abcam
Brand: Abcam Limited
SKU: AB9373
Price: 535 USD
Availability: InStock
Rating: 3 (5 reviews)

(5 Reviews)

(13 Publications)

Rabbit Polyclonal alpha 1 Antitrypsin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human SERPINA1.

View Alternative Names

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB9373)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB9373)

ab9373 staining alpha 1 Antitrypsin in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB9373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling alpha 1 Antitrypsin with ab9373.

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody (AB9373)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody (AB9373)

ICC/IF image of ab9373 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9373, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human SERPINA1.

P01009

Specificity

The antibody is specific against human alpha-1-antitrypsin.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mammals": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

style

left-column

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:875-883 PubMed40065168

2025

Selection for somatic escape variants in SERPINA1 in the liver of patients with alpha-1 antitrypsin deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Brzozowska,Lily Y D Wu,Vera Khodzhaeva,William J Griffiths,Adam Duckworth,Hyunchul Jung,Tim H H Coorens,Yvette Hooks,Joseph E Chambers,Peter J Campbell,Stefan J Marciniak,Matthew Hoare

Methods in molecular biology (Clifton, N.J.) 2750:135-141 PubMed38108974

2024

Autoantibody Recognition of Natural and Homocysteinylated Alpha-1 Antitrypsin: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Quantification in Sera.

Applications

Unspecified application

Species

Unspecified reactive species

Tania Colasanti,Fabrizio Conti

International journal of stem cells 14:475-484 PubMed34711695

2021

Human Liver Stem Cell Transplantation Alleviates Liver Fibrosis in a Rat Model of CCl-Induced Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hyun Lee,Sanghoon Lee,Hey-Jung Park,Young-Ah Kim,Suk-Koo Lee

Nature protocols 16:182-217 PubMed33247284

2020

Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver.

Applications

Unspecified application

Species

Unspecified reactive species

Delilah Hendriks,Benedetta Artegiani,Huili Hu,Susana Chuva de Sousa Lopes,Hans Clevers

Journal of hepatology 71:970-985 PubMed31299272

2019

Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Seon Ju Mun,Jae-Sung Ryu,Mi-Ok Lee,Ye Seul Son,Soo Jin Oh,Hyun-Soo Cho,Mi-Young Son,Dae-Soo Kim,Su Jung Kim,Hyun Ju Yoo,Ho-Joon Lee,Janghwan Kim,Cho-Rok Jung,Kyung-Sook Chung,Myung Jin Son

International journal of molecular sciences 20: PubMed30836679

2019

Endothelial Progenitor Cells as Pathogenetic and Diagnostic Factors, and Potential Targets for GLP-1 in Combination with Metabolic Syndrome and Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Evgenii Germanovich Skurikhin,Olga Victorovna Pershina,Angelina Vladimirovna Pakhomova,Edgar Sergeevich Pan,Vyacheslav Andreevich Krupin,Natalia Nicolaevna Ermakova,Olga Evgenevna Vaizova,Anna Sergeevna Pozdeeva,Mariia Andreevna Zhukova,Viktoriia Evgenevna Skurikhina,Wolf-Dieter Grimm,Alexander Mikhaylovich Dygai

International journal of molecular sciences 20: PubMed30678366

2019

A Retrospective Analysis of the Cartilage Kunitz Protease Inhibitory Proteins Identifies These as Members of the Inter-α-Trypsin Inhibitor Superfamily with Potential Roles in the Protection of the Articulatory Surface.

Applications

Unspecified application

Species

Unspecified reactive species

Susan M Smith,James Melrose

Acta pharmacologica Sinica 40:620-629 PubMed30315254

2018

Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Tang,Ren Guo,Shi-Jun Shen,Yang Zheng,Yu-Ting Lu,Meng-Meng Jiang,Xue Cui,Ci-Zhong Jiang,Xin Xie

World neurosurgery 111:e783-e789 PubMed29309973

2018

Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Ookawa,Masahiko Wanibuchi,Yuko Kataoka-Sasaki,Masanori Sasaki,Shinichi Oka,Shunya Ohtaki,Shouhei Noshiro,Katsuya Komatsu,Yukinori Akiyama,Takeshi Mikami,Nobuhiro Mikuni,Jeffery D Kocsis,Osamu Honmou

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39:1010428317705764 PubMed28618946

2017

A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marut Laohaviroj,Jeremy Potriquet,Xinying Jia,Sutas Suttiprapa,Yaovalux Chamgramol,Chawalit Pairojkul,Paiboon Sithithaworn,Jason Mulvenna,Banchob Sripa

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Antitrypsin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB9373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody (AB9373)

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody (AB9373)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise