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Mouse Monoclonal alpha 1 Antitrypsin antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Full Length Protein corresponding to Human SERPINA1.


Images

Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399), expandable thumbnail
  • Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Full Length Protein corresponding to Human SERPINA1. The exact immunogen used to generate this antibody is proprietary information. Database link P01009

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWBIHC-P
Human
Expected
Tested
Tested

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.Short peptide from AATReversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Alternative names

Recommended products

Mouse Monoclonal alpha 1 Antitrypsin antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Full Length Protein corresponding to Human SERPINA1.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Full Length Protein corresponding to Human SERPINA1. The exact immunogen used to generate this antibody is proprietary information. Database link P01009
Clone number

B9

Purification technique

Affinity purification Protein A

Light chain type

unknown

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR286035-1, GR146648-9 and GR146648-10 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Associated diseases and disorders

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

Product promise

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3 product images

  • Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399), expandable thumbnail

    Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

    Western blot of alpha 1 antitrypsin using ab9399 at a concentration of 0.5 μg/ml.
    lane 2 - human plasma, 2 μl

    lane S - standard proteins.

    A - SDS-PAGE, Coomasie staining

    B - Western blot with ab9399.

    Western blot of alpha 1 antitrypsin using ab9399 at a concentration of 0.5 μg/ml.
    lane 2 - human plasma, 2 μl
    lane S - standard proteins.
    A - SDS-PAGE, Coomasie staining
    B - Western blot with ab9399.

    All lanes: Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

    ab9399 staining human normal liver. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399), expandable thumbnail

    Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

    All lanes: Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) at 1 µg/mL

    All lanes: Western blot - Native human alpha 1 Antitrypsin protein (Active) (Native human alpha 1 Antitrypsin protein (Active) ab91136) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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