AB9399

Anti-alpha 1 Antitrypsin antibody [B9]

Name: Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) | Abcam
Brand: Abcam Limited
SKU: AB9399
Price: 400 USD
Availability: InStock

(2 Reviews)

(21 Publications)

Mouse Monoclonal alpha 1 Antitrypsin antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Full Length Protein corresponding to Human SERPINA1.

View Alternative Names

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399)

ab9399 staining human normal liver. Staining is localized to the cytoplasm.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

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Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399)

Western blot of alpha 1 antitrypsin using ab9399 at a concentration of 0.5 μg/ml.
lane 2 - human plasma, 2 μl
lane S - standard proteins.
A - SDS-PAGE, Coomasie staining
B - Western blot with ab9399.

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399)

Predicted band size: 46 kDa

false

Unknown

Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) at 1 µg/mL

All lanes:

Western blot - Native human alpha 1 Antitrypsin protein (Active) (<a href='/en-us/products/proteins-peptides/native-human-alpha-1-antitrypsin-protein-active-ab91136'>ab91136</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 46 kDa

true

Exposure time: 30s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

ELISA, IHC-P, WB

applications

Immunogen

Full Length Protein corresponding to Human SERPINA1. The exact immunogen used to generate this antibody is proprietary information.

P01009

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR286035-1, GR146648-9 and GR146648-10 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:219 PubMed38710698

2024

CEBPB-mediated upregulation of SERPINA1 promotes colorectal cancer progression by enhancing STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Ma,Ying Chen,Lei Zhan,Qian Dong,Yuanhe Wang,Xiaoyan Li,Lian He,Jingdong Zhang

eLife 12: PubMed36961502

2023

Multi-targeted therapy resistance via drug-induced secretome fucosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Borris D Aldonza,Junghwa Cha,Insung Yong,Jayoung Ku,Pavel Sinitcyn,Dabin Lee,Ryeong-Eun Cho,Roben D Delos Reyes,Dongwook Kim,Soyeon Kim,Minjeong Kang,Yongsuk Ku,Geonho Park,Hye-Jin Sung,Han Suk Ryu,Sukki Cho,Tae Min Kim,Pilnam Kim,Je-Yoel Cho,Yoosik Kim

Journal of molecular histology 53:199-214 PubMed34264436

2021

Zika virus infection of the placenta alters extracellular matrix proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Borges-Vélez,Julio Rosado-Philippi,Yadira M Cantres-Rosario,Kelvin Carrasquillo-Carrion,Abiel Roche-Lima,Jean Pérez-Vargas,Andrea González-Martínez,María S Correa-Rivas,Loyda M Meléndez

Nature communications 10:541 PubMed30710085

2019

MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP.

Applications

Unspecified application

Species

Unspecified reactive species

Yahui Yan,Claudia Rato,Lukas Rohland,Steffen Preissler,David Ron

Clinical proteomics 15:5 PubMed29445323

2018

Urine proteomics of primary membranous nephropathy using nanoscale liquid chromatography tandem mass spectrometry analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Pang,Qianqian Li,Yan Li,Yi Liu,Nan Duan,Haixia Li

International journal of molecular sciences 18: PubMed29244742

2017

Fibrinogen Gamma Chain Mutations Provoke Fibrinogen and Apolipoprotein B Plasma Deficiency and Liver Storage.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Callea,Isabella Giovannoni,Sinan Sari,Esendagli Guldal,Buket Dalgic,Gulen Akyol,Tsuyoshi Sogo,Abdulrahman Al-Hussaini,Giuseppe Maggiore,Andrea Bartuli,Renata Boldrini,Paola Francalanci,Emanuele Bellacchio

Oncotarget 8:113002-113012 PubMed29348884

2017

Alpha-1-antitrypsin functions as a protective factor in preeclampsia through activating Smad2 and inhibitor of DNA binding 4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaling Feng,Nan Wang,Jianjuan Xu,Jinfang Zou,Xi Liang,Huan Liu,Ying Chen

Methods in molecular biology (Clifton, N.J.) 1639:195-209 PubMed28752459

2017

Expression and Purification of Active Recombinant Human Alpha-1 Antitrypsin (AAT) from Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Beena Krishnan,Lizbeth Hedstrom,Daniel N Hebert,Lila M Gierasch,Anne Gershenson

Biomaterials 65:66-75 PubMed26142777

2015

Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice.

Applications

IHC-P

Species

Mouse

Yusuke Sakai,Kosho Yamanouchi,Kazuo Ohashi,Makiko Koike,Rie Utoh,Hideko Hasegawa,Izumi Muraoka,Takashi Suematsu,Akihiko Soyama,Masaaki Hidaka,Mitsuhisa Takatsuki,Tamotsu Kuroki,Susumu Eguchi

Liver international : official journal of the International Association for the Study of the Liver 35:198-206 PubMed24529185

2014

Alpha-1-antitrypsin deficiency: from genoma to liver disease. PiZ mouse as model for the development of liver pathology in human.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Giovannoni,Francesco Callea,Marta Stefanelli,Riccardo Mariani,Filippo M Santorelli,Paola Francalanci

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Antitrypsin antibody [B9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399)

Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399)

Western blot - Anti-alpha 1 Antitrypsin antibody [B9] (AB9399)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

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