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Rabbit Recombinant Monoclonal alpha 1 Antitrypsin antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 12 publications.


Images

Western blot - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (AB207303), expandable thumbnail
  • Western blot - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (AB207303), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (AB207303), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (AB207303), expandable thumbnail
  • Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (AB207303), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/20

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/400

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.Short peptide from AATReversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Alternative names

AAT, PI, PRO0684, PRO2209, SERPINA1, AAT, PI, PRO0684, PRO2209, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

Recommended products

Rabbit Recombinant Monoclonal alpha 1 Antitrypsin antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 12 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17087-50

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Associated diseases and disorders

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303), expandable thumbnail

    Western blot - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303) at 1/5000 dilution

    Lane 1: Human liver lysate at 20 µg

    Lane 2: Human heart lysate at 20 µg

    Lane 3: Human kidney lysate at 20 µg

    Lane 4: Human spleen lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 46 kDa

    Observed band size: 51 kDa, 55 kDa

    Exposure time: 1min

  • Western blot - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303), expandable thumbnail

    Western blot - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303) at 1/5000 dilution

    All lanes: Human fetal liver lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG. at 1/10000 dilution

    Predicted band size: 46 kDa

    Observed band size: 51 kDa, 55 kDa

    Exposure time: 1min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling alpha 1 Antitrypsin with ab207303 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on cancer cells of human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling alpha 1 Antitrypsin with ab207303 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303), expandable thumbnail

    Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)

    alpha 1 Antitrypsin was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab207303 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab207303 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
    Lane 1: HepG2 whole cell lysate 10ug (Input).
    Lane 2: ab207303 IP in HepG2 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207303 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)

    Predicted band size: 46 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling alpha 1 Antitrypsin with ab207303 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse mAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse Alexa Fluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab207303 at 1/50 dilution, followed by Goat Anti-Mouse Alexa Fluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse mAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) seconday antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling alpha 1 Antitrypsin with ab207303 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on K562 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse mAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab207303 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse mAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

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