Rabbit Recombinant Monoclonal alpha 1 Antitrypsin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (AB323289), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-epr26599-43-bsa-and-azide-free-detector-ab323289--sandwich-elisa-img441906.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (AB323289)")
![Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (AB323289), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-epr26599-43-bsa-and-azide-free-detector-ab323289--sandwich-elisa-img441905.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (AB323289)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Alternative names
AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1
Rabbit Recombinant Monoclonal alpha 1 Antitrypsin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26599-43
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab323289 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human alpha 1 Antitrypsin. The recommended pair for sandwich ELISA is:
Capture: Anti-alpha 1 Antitrypsin antibody [EPR26599-26] - BSA and Azide free (Capture) ab323288, Human alpha 1 Antitrypsin Capture Antibody (unconjugated)
Detector: ab323289, Human alpha 1 Antitrypsin Detector Antibody (unconjugated)
The reference range value is 97-12500 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (ab323289), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-epr26599-43-bsa-and-azide-free-detector-ab323289--sandwich-elisa-img441906.jpg "Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (ab323289)")
Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (ab323289)
Interpolated concentration of SERPINA1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: 1/10, 000 dilution Human Plasma and Human Serum. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). We do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (ab323289), expandable thumbnail](https://content.abcam.com/products/images/alpha-1-antitrypsin-antibody-epr26599-43-bsa-and-azide-free-detector-ab323289--sandwich-elisa-img441905.jpg "Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (ab323289)")
Sandwich ELISA - Anti-alpha 1 Antitrypsin antibody [EPR26599-43] - BSA and Azide free (Detector) (ab323289)
Sandwich ELISA of Human alpha 1 Antitrypsin Antibody Pair - BSA and Azide free ab323286 using Human SERPINA1 immunogen protein standard curve with the capture antibody Anti-alpha 1 Antitrypsin antibody [EPR26599-26] - BSA and Azide free (Capture) ab323288 at 2 µg/mL and detector antibody ab323289 dilution at 0.5 µg/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com