AB133642

Anti-alpha 1 Antitrypsin antibody [EPSISR16]

RabMAb
Recombinant
KO Validated
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(3 Publications)

Rabbit Recombinant Monoclonal alpha 1 Antitrypsin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

3 Images

Lab

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (AB133642)

alpha 1 Antitrypsin was immunoprecipitated from 0.35 mg Human plasma 10 μg with 133642 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human plasma 10 μg

Lane 2 : ab133642 IP in Human plasma

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab133642 in Human plasma

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (ab133642)

Predicted band size: 46 kDa

Observed band size: 52 kDa

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Unknown

Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (AB133642)

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (ab133642) at 1/1000 dilution

Lane 1:

Human platelet lysate at 10 µg

Lane 2:

Fetal kidney lysate at 10 µg

Lane 3:

Human plasma lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 46 kDa

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Collaborator

Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (AB133642)

ab133642 was shown to react with SERPINA1 in wild-type HepG2 cells in Western blot with loss of signal observed in a SERPINA1 knockout cell line. Wild-type HepG2 and SERPINA1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133642 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (ab133642) at 1/1000 dilution

Lane 1:

Wild-type HepG2 lysate at 30 µg

Lane 2:

SERPINA1 knock-out HepG2 lysate at 30 µg

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Carrier free

Anti-alpha 1 Antitrypsin antibody [EPSISR16] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPSISR16

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:9925 PubMed31289357

2019

Premedication with a cathepsin C inhibitor alleviates early primary graft dysfunction in mouse recipients after lung transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Salome R T Rehm,Natalia F Smirnova,Carmela Morrone,Jessica Götzfried,Annette Feuchtinger,John Pedersen,Brice Korkmaz,Ali Önder Yildirim,Dieter E Jenne

Journal of translational medicine 16:155 PubMed29879989

2018

Protein markers of dysfunctional HDL in scavenger receptor class B type I deficient mice.

Applications

Species

Mouse

Jia Cao,Yanyong Xu,Feifei Li,Liang Shang,Daping Fan,Hong Yu

BMC musculoskeletal disorders 16:281 PubMed26438379

2015

Comparative study of serum proteomes in Legg-Calve-Perthes disease.

Applications

Species

Unspecified reactive species

Ruiyu Liu,Lihong Fan,Longbin Yin,Kunzheng Wang,Wusheng Miao,Qichun Song,Xiaoqian Dang,Hang Gao,Chuanyi Bai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Antitrypsin antibody [EPSISR16]

Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (AB133642)

Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (AB133642)

Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] (AB133642)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

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Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise