AB54745

Anti-alpha 1 Fetoprotein antibody [1G7]

Name: Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745) | Abcam
Brand: Abcam Limited
SKU: AB54745

(1 Review)

(4 Publications)

Mouse Monoclonal alpha 1 Fetoprotein antibody. Suitable for WB, sELISA, IHC-P, ICC/IF and reacts with Human, Recombinant fragment samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AFP aa 500 to C-terminus.

View Alternative Names

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

alpha 1 Fetoprotein antibody (ab54745) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

This image was generated using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

ab54745 at 10 ug/ml staining alpha Fetoprotein in human HeLa cells by Immunocytochemistry / Immunofluorescence.

This image was generated using the ascites version of the product.

Unknown

Western blot - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

alpha 1 Fetoprotein antibody (ab54745) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)

Predicted band size: 68 kDa

false

Sandwich ELISA - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

sELISA

Unknown

Sandwich ELISA - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

Standard Curve for Alpha 1 Fetoprotein dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to alpha 1 Fetoprotein (ab54745) at 1ug/ml and Detector Antibody Rabbit polyclonal to alpha 1 Fetoprotein (ab8201) at 0.1ug/ml

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1G7

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human AFP aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P02771

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/en-us/products/unavailable/alpha-1-fetoprotein-antibody-ab8201'>ab8201</a> as Detection.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 13 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

See full target information AFP

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed36766855

2023

Pharmacological Targeting of the RAGE-NFκB Signalling Axis Impedes Monocyte Activation under Diabetic Conditions through the Repression of SHP-2 Tyrosine Phosphatase Function.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Dorenkamp,Madina Nasiry,Dilvin Semo,Sybille Koch,Ivonne Löffler,Gunter Wolf,Holger Reinecke,Rinesh Godfrey

ACS applied materials & interfaces 13:8823-8831 PubMed33583183

2021

Metal Nanoparticles/MoS Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for α-Fetoprotein Detection.

Applications

Unspecified application

Species

Unspecified reactive species

Engin Er,Ana Sánchez-Iglesias,Alessandro Silvestri,Blanca Arnaiz,Luis M Liz-Marzán,Maurizio Prato,Alejandro Criado

Communications biology 3:636 PubMed33128033

2020

Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT).

Applications

Unspecified application

Species

Unspecified reactive species

Nan-Ee Lee,Sun Hee Kim,Dae-Yeul Yu,Eui-Jeon Woo,Myung-Il Kim,Gi-Sang Seong,Sun Min Lee,Jeong-Heon Ko,Yong-Sam Kim

Oncology reports 41:2595-2600 PubMed30816512

2019

Inhibition of apoptosis by miR‑122‑5p in α‑fetoprotein‑producing gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Suguru Maruyama,Shinji Furuya,Kensuke Shiraishi,Hiroki Shimizu,Ryo Saito,Hidenori Akaike,Naohiro Hosomura,Yoshihiko Kawaguchi,Hidetake Amemiya,Hiromichi Kawaida,Makoto Sudo,Shingo Inoue,Hiroshi Kono,Daisuke Ichikawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Fetoprotein antibody [1G7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

Western blot - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

Sandwich ELISA - Anti-alpha 1 Fetoprotein antibody [1G7] (AB54745)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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