AB46799

Anti-alpha 1 Fetoprotein antibody

Name: Anti-alpha 1 Fetoprotein antibody (ab46799) | Abcam
Brand: Abcam Limited
SKU: AB46799
Price: 440 USD
Availability: InStock
Rating: 4 (7 reviews)

(7 Reviews)

(49 Publications)

Rabbit Polyclonal alpha 1 Fetoprotein antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse, Cat samples. Cited in 49 publications.

View Alternative Names

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

2 Images

Western blot - Anti-alpha 1 Fetoprotein antibody (AB46799)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab46799 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-alpha 1 Fetoprotein antibody (ab46799) at 1 µg/mL

Lane 1:

HepG2 (Human) Whole Cell Lysate at 10 µg

Lane 2:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 68 kDa

Observed band size: 58 kDa,65 kDa,73 kDa

true

Exposure time: 1min

Unknown

Western blot - Anti-alpha 1 Fetoprotein antibody (AB46799)

All lanes:

Western blot - Anti-alpha 1 Fetoprotein antibody (ab46799) at 1/50000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labeled, at 1/2000 dilution

Predicted band size: 68 kDa

Observed band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Cat, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-fetoprotein also known as alpha 1-fetoprotein or AFP is a glycoprotein composed of a single polypeptide chain with a molecular mass of approximately 69 kDa. AFP is primarily expressed in the fetal liver yolk sac and gastrointestinal tract during embryonic development. In adults the expression of AFP is limited and occurs mainly in the liver. The protein belongs to the albuminoid gene family and shares structural similarity with serum albumin contributing to its role in fetal serum transport.

Biological function summary

Alpha-fetoprotein functions as a fetal plasma protein with roles similar to serum albumin including the binding and transport of ligands such as bilirubin and fatty acids. It does not form part of any larger protein complex but is significant in modulating fetal immune tolerance. In the fetal environment AFP helps protect cells from maternal immune attacks by binding to estrogens and other growth-modulating factors. Its function during pregnancy is critical for maintaining homeostasis and ensuring the fetus's viability.

Pathways

Alpha 1-fetoprotein plays a role in several important biological pathways related to cellular growth and immune response modulation. One such pathway involves regulation of cell proliferation and differentiation in the developing liver. AFP interacts with cellular receptors that influence these processes. Additionally it plays a part in immune pathways interacting with elements that control immune overlooking to fetal antigens hence being linked to both hepatic and immune system proteins like albumin and estrogen receptors.

High levels of alpha-fetoprotein are linked to hepatocellular carcinoma and germ cell tumors such as yolk sac tumor. AFP serves as a tumor marker in these conditions aiding in both diagnosis and monitoring treatment response. In liver cancer AFP levels often correlate with tumor burden while in germ cell tumors it connects to proteins like beta-human chorionic gonadotropin (beta-hCG). Both markers are used together in clinical settings to provide insights into tumor progression and therapeutic efficacy.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

See full target information AFP

style

left-column

Publications (49)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical and translational hepatology 13:555-567 PubMed40937080

2025

CCNE1 Promotes the Progression of Hepatic Precancerous Lesion and the Malignant Phenotype of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Xue Hu,Lichao Yao,Wenzhi Guo

Nature communications 16:6451 PubMed40651942

2025

Androgen receptor promotes arachidonic acid metabolism and angiogenic microenvironment in AFP-negative hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilong Lin,Xiaofei Liu,Houwei Wang,Shumin Li,Ziqiang Miao,Jing Yang,Yuting Zhang,Kai Lei,Yifan Wu,Youmei Kang,Ruoyin Zheng,Zonglin Xie,Yixi Wen,Xiaoxue Ren,Chunxiao Liu,Alfred Sze-Lok Cheng,Yubin Xie,Shuling Chen,Ming Kuang,Sui Peng,Zhenwei Peng,Zihao Dai

Cellular and molecular gastroenterology and hepatology 19:101485 PubMed40024534

2025

Therapeutic Potential of STE20-Type Kinase STK25 Inhibition for the Prevention and Treatment of Metabolically Induced Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xia,Mara Caputo,Emma Andersson,Bernice Asiedu,Jingjing Zhang,Wei Hou,Manoj Amrutkar,Emmelie Cansby,Nadia Gul,Anne Gemmink,Caitlyn Myers,Mariam Aghajan,Sheri Booten,Andrew J Hoy,Anetta Härtlova,Per Lindahl,Anders Ståhlberg,Gert Schaart,Matthijs K C Hesselink,Andreas Peter,Sue Murray,Margit Mahlapuu

Molecular therapy : the journal of the American Society of Gene Therapy 32:4285-4302 PubMed39489916

2024

Reversion of metabolic dysfunction-associated steatohepatitis by skeletal muscle-directed FGF21 gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Jimenez,Victor Sacristan,Claudia Jambrina,Maria Luisa Jaen,Estefania Casana,Sergio Muñoz,Sara Marcó,Maria Molas,Miquel Garcia,Ignasi Grass,Xavier León,Ivet Elias,Albert Ribera,Gemma Elias,Victor Sanchez,Laia Vilà,Alba Casellas,Tura Ferre,Jordi Rodó,Ana Carretero,Marti Pumarola,Marc Navarro,Anna Andaluz,Xavier Moll,Sonia Añor,Sylvie Franckhauser,Mercedes Vergara,Assumpta Caixàs,Fatima Bosch

PloS one 19:e0297285 PubMed38359035

2024

Novel approach for reconstruction of the three-dimensional biliary system in decellularized liver scaffold using hepatocyte progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuya Hirukawa,Hiroshi Yagi,Kohei Kuroda,Masafumi Watanabe,Kotaro Nishi,Shogo Nagata,Yuta Abe,Minoru Kitago,Shungo Adachi,Ryo Sudo,Yuko Kitagawa

Science advances 9:eadh0831 PubMed37703359

2023

Therapeutic blockade of ER stress and inflammation prevents NASH and progression to HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Ebru Boslem,Saskia Reibe,Rodrigo Carlessi,Benoit Smeuninx,Surafel Tegegne,Casey L Egan,Emma McLennan,Lauren V Terry,Max Nobis,Andre Mu,Cameron Nowell,Neil Horadagoda,Darren C Henstridge,Natalie A Mellett,Paul Timpson,Matthew Jones,Elena Denisenko,Alistair R R Forrest,Janina E E Tirnitz-Parker,Peter J Meikle,Stefan Rose-John,Michael Karin,Mark A Febbraio

International journal of cancer 152:2615-2628 PubMed36912275

2023

Transient Kupffer cell depletion and subsequent replacement by infiltrating monocyte-derived cells does not alter the induction or progression of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bart Vanderborght,Kevin De Muynck,Eva Gijbels,Sander Lefere,Charlotte L Scott,Martin Guilliams,Alain Beschin,Mathieu Vinken,Xavier Verhelst,Anja Geerts,Hans Van Vlierberghe,Lindsey Devisscher

Cells 11: PubMed36497057

2022

Long-Term Characteristics of Human-Derived Biliary Organoids under a Single Continuous Culture Condition.

Applications

Unspecified application

Species

Unspecified reactive species

Ranan G Aktas,Michael Karski,Biju Issac,Liang Sun,Shira Rockowitz,Piotr Sliz,Khashayar Vakili

Nature communications 13:3486 PubMed35710796

2022

FUNDC2 promotes liver tumorigenesis by inhibiting MFN1-mediated mitochondrial fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaifeng Li,Shixun Han,Qi Zhang,Yibing Zhu,Haitao Zhang,Junli Wang,Yang Zhao,Jianhui Zhao,Lin Su,Li Li,Dawang Zhou,Cunqi Ye,Xin-Hua Feng,Tingbo Liang,Bin Zhao

Cellular and molecular bioengineering 15:207-217 PubMed35401844

2022

LINC00852 Regulates Cell Proliferation, Invasion, Migration and Apoptosis in Hepatocellular Carcinoma the miR-625/E2F1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha 1 Fetoprotein antibody

Western blot - Anti-alpha 1 Fetoprotein antibody (AB46799)

Western blot - Anti-alpha 1 Fetoprotein antibody (AB46799)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (49)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise